肺水通道蛋白1表达在体外循环致犬肺损伤中的作用  

Role of aquaporin 1 expression in cardiopulmonary bypass-induced lung injury in dogs

作　　者：窦雪娇[1] 张红[1] 韩明[1] 蔡回钧[1] 秦榜勇[1] 王海英[1] 

机构地区：[1]563003遵义医学院附属医院麻醉科

出　　处：《中华麻醉学杂志》2012年第5期555-558,共4页Chinese Journal of Anesthesiology

基　　金：贵州省社发重大攻关项目基金（黔科合SY字[2008]3030）

摘　　要：目的评价肺水通道蛋白1（AQP1）表达在体外循环致犬肺损伤中的作用。方法健康杂种犬24只，体重15～20k，雌雄不拘，采用随机数字表法，将其随机分为4组（n=6）：对照组（c组）、乙酰唑胺I组（AI组）、乙酰唑胺Ⅱ组（AⅡ组）和乙酰唑胺Ⅲ组（AⅢ组），开胸并制备犬CPB模型。C组采用传统预充液，AⅠ组、AⅡ组和AⅢ组分别在预充液中加入乙酰唑胺20、40、60mg／kg。于开胸未行机械通气（T1）、停机（T2）和停机后1h（T3）时取股动脉血，行动脉血气分析，计算呼吸指数（RI）和氧合指数（0I），取肺组织采用RT-PCR及Western—blot法检测AQPlmRNA和蛋白表达，光镜下观察肺组织病理学结果并进行肺损伤评分。结果与C组比较，AⅠ组、AⅡ组和AⅢ组T2.3时P（A—a）O2和RI升高，OI降低，AQP1蛋白表达下调，肺损伤评分升高，AⅡ组和AⅢ组T2，3时AQP1 mRNA表达下调（P〈0．05）；与AⅠ组组比较，AⅡ组和AⅢ组T2，3时P（A—a）O2和RI升高，OI降低，AQP1蛋白表达下调，肺损伤评分升高，AⅢ组T2，时AQP1mRNA表达下调（P〈0．05）；与AⅡ组比较，AⅢ组T2，3时RI升高，AQP1蛋白表达下调，肺损伤评分升高（P〈0．05）。结论AQP1表达下调参与了体外循环致犬肺损伤的过程。Objective To evaluate the role of aquaporin 1 （AQP1） expression in the cardiopulmonary by- pass （CPB）-indueed lung injury in dogs.Methods Twenty-four healthy dogs, weighing 15-20 kg, were randomly divided into 4 group （ n = 6 each） ： control group （group C）, acetazolamideⅠ group （group A Ⅰ ）, acetazolamide Ⅱ group （group A Ⅱ ）, and acetazolamide Ⅲ group （group AⅢ ） . Lung injury was produced by CPB. The traditional priming solution was infused in group C. Priming solutions containing acetazolamide 20, 40 and 60 mg/kg were infused in groups A Ⅰ , A Ⅱ and A Ⅲ respectively. Blood samples were collected from the femoral artery be- fore mechanical ventilation, at the end of CPB and at 1 h after end of CPB （T1-3 ） for arterial blood gas analysis. Respiration index （RI） and oxygenation index （OI） were calculated. The lung specimens were obtained for determination of AQPI mRNA and protein expression （by RT-PCR and Western blot） and for microscopic examination. The pathological changes of the lung were scored. Results Compared with group C, P （A-a） 02, RI and the pathological score were significantly increased at T2,3 , OI was significantly decreased at T2,3 , and AQP1 protein expression was down-regulated at T2,3 in groups A Ⅰ , A Ⅱ and A Ⅱ , and AQP1 mRNA expression was down-regulated at T2,3 in groups A Ⅱ and AⅢ（ P 〈 0.05）. Compared with group A Ⅰ , P（A-a） O2, RI and the pathological score were significantly increased at T2,3 , OI was significantly decreased at T2,3 , and AQPI protein expression was down-regulated at T2.3 in groups A Ⅱ and A Ⅲ, and AQP1 mRNA expression was down-regulated at T2.3 in group AⅢ （ P 〈 0,05） . Compared with group A Ⅱ , RI and the pathological score were significantly increased at T2,3 , and AQP1 protein expression was down-regulated at T2,3 in group AⅢ （ P 〈 0.05） . Conclusion Down-reg- ulation of AQP1 expression is involved in the CPB-induced lung injury in dogs.

关 键 词：水通道蛋白质1 体外循环 呼吸窘迫综合征 成人 

分 类 号：R285.5[医药卫生—中药学]