异丙酚对内脏痛大鼠脊髓背角使君子酸受体GluR1亚基磷酸化水平的影响  

Effects of propofol on phosphorylation of AMPA receptor GIuR1 subunits in a rat model of visceral pain

作　　者：杨昌明[1] 郭瑞娟[2] 王云[2] 时蓉[2] 吴安石[2] 岳云[2] 

机构地区：[1]448000湖北省荆门市第一人民医院麻醉科 [2]首都医科大学附属北京朝阳医院麻醉科

出　　处：《中华麻醉学杂志》2012年第5期563-565,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30801073,81171055）;教育部新世纪优秀人才支持计划（NCET-10-0014）;北京市自然科学基金（7112054）

摘　　要：目的探讨异丙酚对内脏痛大鼠脊髓背角使君子酸（AMPA）受体谷氨酸受体1（GluR1）亚基831和845位点丝氨酸磷酸化水平的影响。方法成年雄性SD大鼠，体重200～300g，6～8周龄，行鞘内置管术，5d后选取鞘内置管成功的大鼠30只，采用随机数字表法，将其随机分为3组（n=10）：假手术组（I组）、内脏痛组（Ⅱ组）和异丙酚组（Ⅲ组）。Ⅱ组和Ⅲ组采用经直肠注射50μl 10％辣椒素的方法制备内脏痛模型，I组给予等容量生理盐水，Ⅲ组于给予辣椒素前10min鞘内注射异丙酚20μg，Ⅰ组和Ⅱ组给予等容量二甲基亚砜。记录给予辣椒素后30min内大鼠累计痛评分，然后处死大鼠，取L3-6腰骶段脊髓背角组织，采用免疫印迹法检测AMPA受体GluR1亚基及其831和845位点丝氨酸磷酸化水平。结果与Ⅰ组比较，Ⅱ组和Ⅲ组累计痛评分和脊髓背角GluR1亚基831和845位点丝氨酸磷酸化水平升高（P〈0．05或0．01），而GIuR1亚基表差异无统计学意义（P〉0．05）；与Ⅱ组比较，Ⅲ组累计痛评分和脊髓背角GluR1亚基831和845位点丝氨酸磷酸化水平降低（P〈0．05或0．01）。结论异丙酚可减轻大鼠内脏痛，其机制与抑制脊髓背角AMPA受体GluR1亚基831和845位点丝氨酸磷酸化有关。Objective To investigate the effects of propofol on the phosphorylation of α-amino-3-hydroxy- 5-methyl-4-isoxazolepropionate （AMPA） receptor GluR1 subunits at Serine-831 and Serine-845 sites in the spinal cord dorsal horn in a rat model of visceral pain. Methods Thirty male SD rats, aged 6-8 weeks, weighing 200- 300 g, in which intrathecal catheters were successfully placed without complications, were randomly divided into 3 groups （ n = 10 each） ： sham operation group （group Ⅰ ）, visceral pain group （group Ⅱ ） and propofol group （group Ⅲ ） . Visceral pain was induced by injection of 10% capsaicin 50 μl via the rectum in groups Ⅱ and Ⅲ. While the equal volume of normal saline was given instead in group Ⅱ . Group Ⅲ received intrathecal injection of propofol 20 μg at 10 min before injection of capsaicin. While the equal volume of dimethyl sulfoxide was given instead of propofol in groups Ⅰ and Ⅱ . The cumulative pain score was recorded during 30 min after capsaicin injection. The rats were then sacrificed, and the lumbar segment （ L3.6 ） of the spinal cord was removed for determina- tion of the expression of GluR1 subunits and phosphorylation of GluR1 subunits at Serine-831 and Serine-845 sites in the spinal cord dorsal horn. Results Compared with group I , the cumulative pain score and phosphorylation of GluR1 subunits at Serine-831 sites and Serine-845 in the spinal cord dorsal horn were significantly increased （ P 〈0.05 or 0.01）, but there were no significant differences in the expression of GluRI subunits in groups Ⅱ and Ⅲ（ P 〉 0.05 ）, Compared with group Ⅱ , the cumulative pain score and phosphorylation of GluR1 subunits at Serine- 831 and Serine-845 sites in the spinal cord dorsal horn were significantly decreased in group Ⅲ （ P 〈 0.05 or 0.01 ）. Conclusion Propofol can attenuate the visceral pain through the inhibition of the phosphorylation of AMPA receptor GIuR1 subunits at Serine-831 and Serine-845 sites in the rat spinal cord dorsal

关 键 词：二异丙酚 受体 AMPA 脊髓 疼痛 

分 类 号：R965[医药卫生—药理学]