O-GlcNAc修饰在谷氨酰胺减轻脓毒症大鼠脑损伤中的作用  

Role of O-GIcNAc protein modification in attenuation of brain damage by gintamine in septic rats

作　　者：王瑾[1] 陆新健[1] 郑康[1] 景亮[1] 

机构地区：[1]210009南京市,东南大学附属中大医院麻醉科

出　　处：《中华麻醉学杂志》2012年第5期613-615,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价O-GlcNAc修饰在谷氨酰胺减轻脓毒症大鼠脑损伤中的作用。方法健康清洁级4周龄sD雄性大鼠60只，体重180～240g，采用随机数字表法，将其随机分为4组：假手术组（s组，n=12）、脓毒症组（CLP组，n=16）、谷氨酰胺组（G组，n=16）及谷氨酰胺＋四氧嘧啶组（G＋A组，n=16）。采用盲肠结扎穿孔术（CLP）建立大鼠脓毒症模型，G组术毕静脉注射谷氨酰胺0．75g／kg，G＋A组术毕静脉注射谷氨酰胺0．75g／kg及腹腔注射四氧嘧啶90mg／kg，CLP组和s组注射等容量生理盐水。术后24h时记录神经反射评分，断颈处死，测定脑组织含水量，采用Western—blot法测定脑组织O-GlcNAc表达水平，观察脑组织病理学结果。结果与s组比较，CLP组、G组及G＋A组大鼠脑组织含水量、神经反射评分升高（P〈0．05）；与CLP组比较，G组和G＋A组大鼠脑组织含水量、神经反射评分降低，G组大鼠脑组织蛋白质O-GlcNAc表达上调（P〈0．05）；与G组比较，G＋A组大鼠脑组织含水量、神经反射评分升高，脑组织蛋白质O-GlcNAc表达下调（P〈0．05）；S组、CLP组及G＋A组大鼠间脑组织蛋白质O-GlcNAc表达差异无统计学意义（P〉0．05）。G组病理学损伤较CLP组及G＋A组轻。结论O-GlcNAc修饰参与了谷氨酰胺减轻脓毒症大鼠脑损伤的过程。Objective To evaluate the role of O-GlcNAc protein modification in attenuation of brain damage by glutamine in septic rats. Methods Sixty male SD rats weighing 180-240 g were randomly divided into 4 groups： sham operation group （group S, n = 12）, sepsis group （group CLP, n = 16）, glutamine group （group G, n = 16）, an inhibitor of O-linked-N-acetyl glucosamine transferase Alloxan ＋ glutamine group （group G ＋ A, n = 16） . Rats were submitted to sepsis by cecal ligation and perforation （CLP） . Glutamine （Gln） 0.75 g/kg was injected iv after CLP in group G. Gln 0.75 g/kg was injected iv and Alloxan 90 mg/kg was injected ip after CLP in group G ＋ A. Equal volume of normal saline was given in group S and group CLP. At 24 h after CLP, the neural reflex score was evaluated, then rat was sacrificed. The brain was removed for measurement of brain water content, observation of histopathology and determination of O-GlcNAe-modified protein expression. Results Compared with group S, neural reflex score and brain water content were significantly increased in groups CLP, G and G ＋ A （ P 〈 0.05）. Compared with group CLP, neural reflex score and brain water content were significantly decreased in groups G and G ＋ A （ P 〈 0.05）, and the expression of O-GlcNAc-modified protein was upregulated in group G （ P 〈 0. 05）, Compared with groups G, neural reflex score and brain water content were significantly increased, and the expression of O-GleNAe-modified protein downregulated in group G ＋ A （P 〈 0.05 ）. There was no significant difference in O-GlcNAc-modified protein expression among groups S, CLP and G ＋ A. Conclusion Glutamine attenuates brain damage through O-GlcNAc protein modification in septic rats.

关 键 词：谷氨酰胺 脓毒症 O-GlcNac修饰 脑 

分 类 号：R459.7[医药卫生—急诊医学]