快速测定不同市售补骨脂药材中补骨脂素、异补骨脂素的含量  

Determination of Psoralen and Isopsoralen in Psoralea Corylifolia from Different Commercially Available

作　　者：李凯[1] 张太行[1] 周宁[1] 贾利利[1] 

机构地区：[1]河南中医学院药学院,河南郑州450008

出　　处：《中医学报》2012年第7期846-848,共3页Acta Chinese Medicine

基　　金：河南中医学院博士基金项目(编号:BSJJ-201006)

摘　　要：目的:建立简便、快速的检测补骨脂药材中补骨脂素和异补骨脂素含量的方法,并测定不同市售补骨脂药材中补骨脂素、异补骨脂素含量。方法:采用C18ODS色谱柱(1.8μm,4.6 mm×50.0 mm),流动相乙腈-水(3565),流速0.5 mL.min-1,检测波长246 nm。结果:5 min内补骨脂素、异补骨脂素分离良好。在相应浓度范围内2种化合物的线性关系良好,r>0.999 7;精密度RSD≤2.5%;平均加样回收率为98%。结论:该方法节省了分析时间和溶剂,为补骨脂中补骨脂素、异补骨脂素的分析提供了一种简便快速的新手段。Objective：To establish a simple,rapid detective method of psoralen and isopsoralen in Malaytea Scurfpea,and determinate their contents in different commercial Malaytea Scurfpea.Methods：C18 ODS column（1.8 μm,4.6 mm×50 mm） was adopt,the mobile phase was composed of acetonitrile and water（3565） at flow rate of 0.5 mL/min,detective wavelength was 246 nm.Results：Psoralen and Isopsoralen was well separated in 5 min.The two kinds of compounds was well linear correlated within corresponding concentration range,r0.9997;RSD≤2.5 %;average recovery98%.Conclusion：The method significantly saved analysis time and solvent,and provided a simple,fast means for determination of psoralen and isopsoralen.

关 键 词：补骨脂 补骨脂素 异补骨脂素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]