姜黄素对癫痫小鼠海马脑损伤的保护作用  

The protective effect of curcumin on hippocampus of epileptic mouse

作　　者：孟伟 杨锦青 鞠培新 贾本智[3] 李洪秀 

机构地区：[1]盘锦市第二人民医院脑外科,辽宁盘锦124000 [2]辽河油田中心医院,辽宁盘锦124000 [3]中国医科大学附属第四医院感染科,辽宁沈阳110001

出　　处：《解剖科学进展》2012年第4期323-325,328,共4页Progress of Anatomical Sciences

基　　金：盘锦市科技计划项目(No.20080732)

摘　　要：目的研究姜黄素对匹罗卡品诱导的癫痫小鼠海马新生神经元和细胞凋亡的影响。方法姜黄素预处理后,小鼠腹腔注射匹罗卡品建立小鼠癫痫模型,应用新生神经元标记物双皮层蛋白(doublecortin,DCX)免疫组织化学染色及TUNEL染色对造模后72h的模型小鼠海马进行检测。结果 DCX免疫组织化学染色结果表明,与对照组相比,模型组及姜黄素处理模型组小鼠海马齿状回DCX阳性细胞明显减少;与模型组相比,姜黄素处理模型组小鼠海马齿状回DCX阳性细胞明显增多。TUNEL染色结果表明,与对照组相比,模型组及姜黄素处理模型组小鼠海马齿状回TUNEL阳性细胞明显增多;与模型组相比,姜黄素处理模型组小鼠海马齿状回TUNEL阳性细胞明显减少。结论姜黄素可能对匹罗卡品诱导的癫痫小鼠海马神经元有保护作用。Objective To investigate the effects of curcumin on newborn neurons and apoptosis in epileptic mouse hippoeampus. Method Experimental animals （pre-treatment with curcumin） were injected （i.p.） with a dose of 300 mg/kg of pilocarpine to induce seizure, 72 h after pilocarpine treatment, doublecortin （DCX） immunohistoehemistry and TUNEL staining were used to detect newborn neurons and apoptosis in epileptic hippocampus. Results Compared with controls, there were less DCX positive cells in granular cell layer of the model group and curcumin treated model group, whereas more DCX positive cells in curcumin treated model group than in model group. TUNEL staining indicated that less TUNEL positive cells in the dentate gyrus （DG） were found in curcumin treated model group than in model group. Conclusion Curcumin has a protective effect on hippocampus neurons of the pilocarpine-induced epileptic mouse.

关 键 词：癫痫 姜黄素 小鼠 

分 类 号：R338.26[医药卫生—人体生理学]