hUCMSCs腹腔移植治疗大鼠急性肝损伤后的病理变化  

Effect of hUCMSCs celiac transplantation on the acute rats hepatic injury

作　　者：郑学峰[1] 刘霞[2] 

机构地区：[1]辽宁医学院附属第一医院消化内科,辽宁锦州121001 [2]辽宁医学院组织与胚胎学教研室,辽宁锦州121001

出　　处：《解剖科学进展》2012年第4期345-348,共4页Progress of Anatomical Sciences

基　　金：辽宁省教育厅资助项目(L2010296)

摘　　要：目的本研究拟对CCl所致大鼠急性肝损伤模型应用人脐带间充质干细胞(humanum bilical cord mesenchymal stem cells,hUCMSCs)进行治疗,观察其效果。方法将健康雌性150-170gSD大鼠随机分为3组:正常对照组(N,n=8),CCl_4组(C,n=32)及CCl_4+MSCs组(T,n=32)。N组不予任何处理;T组和C组腹腔注射10%(V/V)四氯化碳(carbon tetrachloride,CCl_4)橄榄油溶液制造急性肝损伤模型后24h,分别经腹腔移植hUCMSCs和等量PBS。肝功能测定、病理组织学方法观察移植前后大鼠肝功能及肝脏结构改善情况。结果 CCl腹腔注射24h后大鼠肝脏的HE染色示弥漫性肝小叶中心带肝细胞气球样变或脂肪变性,可见炎性细胞浸润。4随着观察时间的推移,上述组织学变化均好转,T组较C组更早更快的恢复,7d时肝脏结构完全恢复正常,C组7d时仍可见脂滴或气球样变,14d才恢复正常肝脏组织结构。MSCs移植后3d,T组与C组比较,肝损伤大鼠的ALT(p<0.05)及AST(p<0.01)水平明显降低,说明MSCs移植可降低CCl_4所致大鼠肝脏损伤的ALT及AST水平。47d后,所有观察组的ALT及AST均降至正常水平。结论鼠腹腔移植hUCMSCs后可以在一定程度上改善肝功能及修复受损的肝组织。Objective To investigate the treatment effect of human umbilical cord mesenchymal stem cells（hUCMSCs ） on rats acute liver damage model caused by CC14. Methods Rats were divided into 3 groups： normal control （N, n=lO）, CC14 （C, n=10） and CC14 plus MSCs （T, n=10）. All the rats were sampled at the appointed times and various target markers were determined as follows： HE staining, the index changes of liver＇s enzymology to evaluate the improvement of hepatic histological damage. Results Ballooning degeneration or fatty degeneration hepatic cells were observed in diffuse eentrilobule by HE dyeing 24h after CC14 by intraperitoneal injection of rats. As the observation time proloned, the above histologic changes were improved and recovered more earlier and faster in T group than in C group. The levels of ALT （P〈0.05） and AST （P〈O.01） were obviously increased in C group than in N group, but reduced in T group than in C group, downregulated to normal levels in all groups 7d after damage of liver. Conclusion hUCMSCs transplantation can repair the injured liver function and histological structure induced by CC14.

关 键 词：脐带间充质干细胞 急性肝损伤 大鼠 丙氨酸转氨酶 天门冬氨酸转氨酶 

分 类 号：Q291[生物学—细胞生物学]