早期自然流产妇女血小板活化因子及其受体的表达  

Expressions of platelet activating factor and its receptor in the women with early spontaneous abortion

作　　者：王亚芹[1] 刁英[1] 

机构地区：[1]遵义医学院第一附属医院生殖中心,贵州遵义563003

出　　处：《中国妇幼保健》2012年第19期2999-3002,共4页Maternal and Child Health Care of China

基　　金：贵州省遵义医学院基金项目〔(2006)06〕

摘　　要：目的:探讨血小板活化因子(platelet-activating factor,PAF)及其受体(PAF-Receptor,PAF-R)与早期自然流产的关系。方法:30例早期自然流产妇女(研究组)和30例同期正常早孕要求人工流产的妇女(对照组)采用ELISA法检测血清PAF的含量,采用流式细胞仪检测外周血单核细胞上PAF-R的表达,应用免疫组织化学技术检测PAF-R在子宫蜕膜组织中的表达。结果:①研究组血清中PAF含量、PAF-R在外周血单核细胞上和在子宫蜕膜组织中的表达与对照组比较,差异有统计学意义(P<0.01)。②早期自然流产妇女PAF-R在外周血单核细胞上和在子宫内膜上的表达呈正相关(r=0.938,P<0.01)。结论:早期自然流产妇女血清PAF水平明显降低,PAF-R在外周血单核细胞上和子宫蜕膜组织中的表达降低,可能是导致自然流产的原因之一。外周血单核细胞上PAF-R的表达可间接反映子宫蜕膜组织中PAF-R的表达情况。Objective： To explore the relationship between platelet activating factor： （PAF）, platelet activating factor receptor （ PAF - R） and early spontaneous abortion. Methods ： The contents of serum PAF in 30 women with early spontaneous abortion （ study group） and 30 women with normal early pregnancy and requiring artificial abortion （control group） were detected by ELISA, flow cytometry was used to detect the expression of PAF -R in peripheral blood monocytes （PBMCs） ; the expression of PAF- R in decidual tissue was detected by immunohistochemical method. Results ： There was statistically significant difference in serum content of PAF, the expression levels of PAF - R in PBMCs and decidual tissue between study group and control group （P 〈 0.01 ） . There was a positive correlation between PAF - R expression in PBMCs and PAF - R expression in decidual tissue in study group （ y = 0. 938, P 〈 0.01 ） . Conclusion ： The serum level of PAF and the expression levels of PAF - R in PBMCs and decidual tissue in the women with early spontaneous abortion decreased, which may be one of the causes of spontaneous abortion. PAF - R expression in PBMCs can indirectly reflect the expression of PAF - R in decidual tissue.

关 键 词：自然流 产血小板活化因子 受体 妊娠早期 

分 类 号：R714.21[医药卫生—妇产科学]