山茱萸水提液对人胚肺成纤维细胞(MRC-5)的影响机制初探  

The Effect Mechanism of Cornus Extraction on Human Embryonic Lung Fibroblast Cells (MRC-5)

作　　者：李桂霞[1] 高书亮[1] 赵凤鸣[2] 许冬青[2] 皮文霞[2] 徐德国[2] 周韬[2] 王明艳[2] 

机构地区：[1]江西中医学院,江西南昌330006 [2]南京中医药大学,江苏南京210029

出　　处：《时珍国医国药》2012年第6期1361-1363,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30772851)

摘　　要：目的探讨山茱萸水提液体外对D-半乳糖致衰人胚肺成纤维细胞(MRC-5)的影响作用及机制。方法 MTT法观察山茱萸水提液对D-半乳糖致衰MRC-5细胞生长状况的影响;Western blot法观察山茱萸水提液对D-半乳糖致衰MRC-5细胞抗衰老通路P16,P21的影响;Telomere PNA Kit/FITC for Flow Cytometry法观察山茱萸水提液对MRC-5细胞端粒长度的影响。结果 MTT检测山茱萸水提液对D-半乳糖致衰MRC-5细胞有促进增殖作用;Western blot法检测,与对照组比较山茱萸水提液能够使D-半乳糖致衰MRC-5细胞P16、P21表达降低;与对照组比较山茱萸水提液能够延缓端粒缩短。结论山茱萸水提液有抗衰老作用。Objective To study the effects and mechanisms of Comus water extraction in human embryonic lung fibroblast cells （ MRC -5 ） induced aging by D - galactose. Methods To observe growth conditions by MTI＇ method about the water extraction of Comus on human embryonic lung fibroblast cells （ MRC - 5 ） induced aging by D - galaetose ; To detect anti - aging pathway of P16, P21 in Western blot about Comus water extraction on MRC - 5 cells induced aging by D - galactose. In experiment of Telomere PNA Kit / FITC for Flow Cytometry , study telomere length influences of Comus water extraction on MRC - 5 cell. Results The results ofMTY method showed the water extraction of Comus can promote reproduce of human embryonic lung fibroblast cells （ MRC - 5） in- duced aging by D -galactose ;Western blot method and Telomere PNA Kit/F1TC for Flow Cytometry results reveal , compared with the control group,Comus water extraction could make P16 and P21 expression reduced of MRC -Scell induced aging by D - galac- tose; and delay the telomere shortening of MRC - 5 cell. Conclusion Comus water extraction have anti - aging function.

关 键 词：山茱萸 衰老 P16 P21 蛋白印迹 端粒 

分 类 号：R285.5[医药卫生—中药学]