当归红芪超滤物对自然衰老大鼠抗氧化作用机制的研究  

The Principle of Anti-age Effects on Naturally Aged Mice from Ultra-filtration Extract Mixture from Angelica Sinensis and Hedysarum Polybotrys

作　　者：李应东[1,2] 王博雯[1] 周倩倩[1] 刘凯[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000 [2]甘肃中医学院附属医院,甘肃兰州730000

出　　处：《时珍国医国药》2012年第6期1439-1441,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家教育部"春晖计划"(No.Z2004-1-62042);国家自然科学基金(No.81160478/H2902)

摘　　要：目的探讨当归红芪超滤物的抗衰老作用及其机制并比较不同分子量超滤物的作用效果。方法将42只Wistar老年雄性大鼠随机分为衰老对照组和当归红芪超滤膜提取物20万、10万、5万分子量组,给药组连续灌胃10周后,按试剂盒说明书测定血清及心肌组织中SOD、GSH-Px的活力及MDA含量水平。结果与衰老对照组相比,当归红芪超滤膜提取物20万、10万、5万分子量组的血清及心肌组织中SOD、GSH-Px活力水平不同程度提高(P<0.05),MDA水平不同程度降低(P<0.05);不同分子量组间比较,10万分子量组的SOD、GSH-Px的活性水平提高最显著(P<0.05),且MDA含量水平降低最显著(P<0.05)。结论当归红芪超滤物抗衰老的作用机制可能与提高心肌组织中的SOD、GSH-Px活力和降低MDA含量从而发挥抗氧化作用相关,其抗氧化的有效成分可能在10万分子量上下。Objective To discuss the anti - age effect of ultra - filtration extract mixture from Angelica sinensis and Hedysarum polybotrys and its working principles and to compare the results generated with different ultra - filtration extract of molecular weight. Methods Randomly divided 42 Wistar aged male mice into aging coutrol group and 200 000,100 000,50 000 molecular weight groups. After 10 weeks of continuous stomach irrigating to the medical group, we measured the activity of SOD, GSH - Px in and quantity level of MDA serum and myocardial tissue, according to the manual written on reagent containers. Results By compa- ring with aging control group, the activity levels of SOD, GSH - PX in serum and myocardial tissue of different molecular weight groups have increased individually（ p 〈 0.05 ）. MDA levels have decreased individually （P 〈 0.05 ）. Among different molecular weight groups, the SOD, GSH - Px activity level of 100 000 molecular weight group had the most prominent increase （ P 〈 0.05 ）. And the this group also had the most noticeable decrease in MDA level changes （P 〈 0.05 ）. Conclusion The principle of anti - age effect from ultra - filtration extract mixture from Angelica sinensis and Hedysarum polybotrys can be affected by increasing the activity level of SOD, GSH - Px and decreasing the quantity of MDA in myocardial tissues. The effective anti - age elements can be around 100 000 molecular weight.

关 键 词：当归红芪超滤物 衰老 抗氧化 超氧化物歧化酶 谷胱甘肽-过氧化物酶 丙二醛 

分 类 号：R284.2[医药卫生—中药学]