Structural insights into DndE from Escherichia coil B7A involved in DNA phosphorothioation modification  

作　　者：Wei Hu Chengkun Wang Jingdan Liang Tianlong Zhang Zhongpei Hu Zhijun Wang Wenxian Lan Fang Li Houming Wu Jianping Ding Geng Wu Zixin Deng Chunyang Cao 

机构地区：[1]State Key Laboratory of Bio-organic and Natural Product Chemistry,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,345 Lingling Road,Shanghai 200032,China [2]Labo-ratory of Microbial Metabolism,and School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200030,China [3]Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Shanghai 200031,China [4]Central China Normal University,Wuhan,Hubei 430079,China

出　　处：《Cell Research》2012年第7期1203-1206,共4页细胞研究（英文版）

基　　金：Acknowledgments This work was supported by National Basic Research Program of China （2009CB918600 and 2011 CB966300）, National Natural Science Foundation of China （20872169, 20905074 and 20921091）, National New Drug Design Program from Ministry of Health of China （2011ZX09506）, and Pujiang Talents Awards from Science and Technology Commission of Shanghai Municipality （08P J14117）. We thank Prof Jianhua He at the beamline BL 17U at SSRF （China） for assistance in data collection.

摘　　要：Dear Editor, DNA phosphorothioate （PT） modification, originally developed as an artificial tool to stabilize oligodeoxynucleotides against nuclease degradation , was recently found to be incorporated with sulfur into DNA backbone as a novel physiological variation by the five-gene dnd cluster （dndA-dndE） products in a sequence- and stereo-specific manner .

关 键 词：DNA 大肠杆菌 修改 结构 寡核苷酸 生理变化 磷酸酯 酶降解 

分 类 号：Q523[生物学—生物化学] Q78