Serum inducible kinase is a positive regulator of cortical dendrite development and is required for BDNF-promoted dendritic arborization  

作　　者：Shun-Ling Guo Guo-He Tan Shuai Li Xue-Wen Cheng Ya Zhou Yun-Fang Jia Hui Xiong Jiong Tao Zhi-Qi Xiong 

机构地区：[1]Institute of Neuroscience and State Key Laboratory of Neuroscience,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Yueyang Road#320,ION building,Room 426,Shanghai 200031,China [2]Xinhua Hospital School of Medicine,Shanghai Jiao Tong University,Shanghai 200092,China

出　　处：《Cell Research》2012年第2期387-398,共12页细胞研究（英文版）

摘　　要：Serum inducible kinase （SNK）, also known as polo-like kinase 2 （PLK2）, is a known regulator of mitosis, synapto- genesis and synaptic homeostasis. However, its role in early cortical development is unknown. Herein, we show that snk is expressed in the cortical plate from embryonic day 14, but not in the ventricular/subventricular zones （VZ/ SVZ）, and SNK protein localizes to the soma and dendrites of cultured immature cortical neurons. Loss of SNK im- paired dendritic but not axonal arborization in a dose-dependent manner and overexpression had opposite effects, both in vitro and in vivo. Overexpression of SNK also caused abnormal branching of the leading process of migrating cortical neurons in electroporated cortices. The kinase activity was necessary for these effects. Extracellular signal- regulated kinase （ERK） pathway activity downstream of brain-derived neurotrophic factor （BDNF） stimulation led to increases in SNK protein expression via transcriptional regulation, and this upregulation was necessary for the growth-promoting effect of BDNF on dendritic arborization. Taken together, our results indicate that SNK is essen- tial for dendrite morphogenesis in cortical neurons.

关 键 词：PLK2 MORPHOGENESIS BDNF ERK transcription 

分 类 号：Q421[生物学—神经生物学] Q55[生物学—生理学]