夫西地酸产生菌菌落形态与代谢特征的研究  

Study on the relationship between colonial morphology of Fusidium coccineum and metabolic characteristics

作　　者：邓昕[1] 蓝鸿[2] 董晓景[2] 李继安[2] 陈代杰 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040 [3]中国医药工业研究总院,上海200040

出　　处：《上海师范大学学报（自然科学版）》2012年第3期312-318,共7页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(81072557);国家自然科学基金(81172962);上海市青年科技启明星计划(B类)(11QB1406300);国家"重大新药创制"科技重大专项(2010ZX09401-403)

摘　　要：通过对梭链孢脂球菌SIPI-A.3201进行紫外诱变处理,挑选出3种比较典型的单菌落.摇瓶发酵考察它们的生长代谢特性,检测菌体浓度、糖氮代谢、pH变化以及夫西地酸生物合成量等几项指标.并且对菌株Ⅰ和菌株Ⅲ发酵过程中的菌丝形态变化进行了研究.结果表明:与菌株Ⅰ和菌株Ⅱ相比,菌株Ⅲ草帽型的单菌落产抗水平最高,达到了约480×10-6g/mL.Three mutants having typical colonies with different morphology of Fusidium coccineum SIPI-A. 3201 named I,II and III in this paper were obtained in the process of strain improvement by UV mutation. Metabolic characteristics of them, such as cell growth, changes of pH,nitrogen and sugar,and the biosynthesis of fusidic acid, were measured in the course of fermentation in shaking-flask. The result showed that the mutant with typical morphology of colony m biosynthezised highest fusidic acid,up to 480 × 10-6g/mL.

关 键 词：夫西地酸 菌落形态 代谢特性 

分 类 号：Q815[生物学—生物工程]