姜黄素预处理对大鼠肾脏缺血再灌注损伤的预防效果及机制探讨  

Protective effect and mechanism of curcumin preconditioning on renal isehemia reperfusion injury in rats

作　　者：陶佳意[1] 米华[1] 莫曾南[1] 黄伟华[1] 梁艺耀[1] 黄海鹏[1] 李生华[1] 陈凯[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《山东医药》2012年第22期1-3,共3页Shandong Medical Journal

基　　金：广西壮族自治区科技厅留学回国人员基金资助项目(0832010)

摘　　要：目的观察姜黄素预处理预防大鼠,肾脏缺血再灌注损伤(IRI)的效果,并探讨其机制。方法 36只SD雄性大鼠随机分为3组,分别为假手术组(Sham组)、肾脏缺血再灌注模型组(IR组)、姜黄素预处理组(CUR组),每组12只。IR组和CUR组先切除右肾,游离左肾蒂,钳夹左肾蒂45 min后放开。CUR组在钳夹左肾蒂前2 h给予姜黄素100 mg/kg溶于0.1%二甲基亚砜1 mL中,注入腹腔。再灌注24 h后沿原切口进入,分别取下腔静脉血和切除左肾。下腔静脉血用于测定血清肌酐(Cr)和尿素氮(BUN)。取左肾组织匀浆采用硫代巴比妥酸法测定丙二醛(MDA)含量,采用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性,并行HE染色观察各组肾组织结构变化。结果与Sham组比较,IR组和CUR组血清Cr、BUN均升高(P均<0.05)。与IR组比较,CUR组的血清Cr、BUN值在再灌注24 h后均下降(P均<0.05)。与Sham组比较,IR组肾脏组织匀浆SOD活性明显降低,MDA含量明显升高(P均<0.05)。与IR组比较,CUR组肾脏组织匀浆SOD活性升高,MDA含量降低(P均<0.05)。肾组织HE染色后光镜下观察可见与Sham组比较,IR组肾小管损伤明显;与IR组比较,CUR组肾小管损伤减轻明显。结论姜黄素预处理对大鼠IRI有预防作用。其机制可能与姜黄素减轻IRI大鼠肾脏氧化应激水平有关。Objective To investigate the effect and mechanism of curcumin preconditioning on the renal ischemia- reperfusion injury （IRI） of rats. Methods Thirty six SD rats were randomly divided into three groups, namely sham-oper- ated group（ group Sham）, renal ischemia reperfusion model group（ group IR）, and eureumin preconditioning group（ group CUR） , there were 12 rats in each group. In group IR and group CUR, the right kidney were removed, the left renal pedi- eles were clamped for 45 min and reperfused for 24 h. In group CUR, cureumin were given before 2 hours of surgery, ac- cording to the dose that 100mg/kg dissolved in 1 ml of 0.1% dimethyl sulfoxide （DMSO）, injected into the peritoneal cavi- ty, and then the left kidney was treated for 45 minutes isehemia and reperfused for 24 hours. After reperfusion for 24 hours, open the original incision, the inferior vena venous blood was extracted and the left kidney was removed. Determin the blood creatinine （Cr） and urea nitrogen （ BUN ）. Superoxide dismutase （SOD） activity and malondialdehyde （ MDA ） value were measured in renal tissue, and hematoxylin eosin（HE） staining observe the changes in renal structure. Results Compared to group Sham, the values of Cr, BUN in group IR and group CUR were significantly high （ P 〈 0.05 ）. Com- pared to the group IR, the group CUR Cr, BUN values significantly decreased after reperfusion for 24 h （P 〈 0.05 ）. Com- pared to group Sham, group IR SOD activity decreased, while the value of MDA increased （P 〈 0.05 ）. Compared to group IR, group CUR had higher SOD activity, while the value of MDA decreased. Observed with optical microscopy, compared to group Sham, the renal tubular injury of group IR was obvious. Compared to group IR, group CUR signifi-cantly reduced tubular injury. Conclusion Curcumin preconditioning can protect the kidney from IRI. Curcumin can re- duce IRI kidney oxidative stress, may be one of the mechanisms.

关 键 词：肾脏 缺血再灌注损伤 姜黄素 丙二醛 超氧化物歧化酶 大鼠 

分 类 号：R692[医药卫生—泌尿科学]