模拟不同海拔高原环境下氦氧潜水对人血清丙二醛含量和促红细胞生成素及抗氧化酶活力的影响  

Effects of simulated heliox diving on serum malondialdehyode levels and activities of erythropoietin and antioxidases of the divers at different high altitudes

作　　者：杨晨[1] 胡慧军[1] 张良[1] 张禹[1] 潘晓雯[1] 

机构地区：[1]海军总医院全军高压氧治疗中心,北京100048

出　　处：《中华航海医学与高气压医学杂志》2012年第3期135-137,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：总装备部装备技术基础项目（YXSJD20101BZ01）

摘　　要：目的检测模拟高原潜水实验中潜水员血清氧化损伤产物含量以及促红细胞生成素和抗氧化酶系活性变化，探讨高海拔潜水对人体氧化损伤的影响。方法模拟高原潜水实验人员进舱前、潜水停留过程中、潜水结束后及出舱后采静脉血，检测血清丙二醛（MDA）含量、促红细胞生成素（EPO）水平、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性以及总抗氧化活性（TAOC）。结果实验前、中、后MDA含量在[（3．314±1．35）-（6．98±3．52）]μmol／L范围内波动，各种抗氧化酶活性水平基本在正常范围内。血清MDA含量、GSH-Px活性与环境氧分压及暴露时间存在相关性，在高氧·低氧交替变化的环境中血清EPO水平与SOD和CAT呈反向相关趋势。结论本次实验条件下低一高氧环境的反复变化未对4名潜水员造成可检测到的氧化损害。Objective To observe the levels of serum oxidative products （MDA） and changes in the activities of antioxidases in the simulated high altitude dive, and also to study the effects of the high altitude dive on oxidative damage to the body. Methods Blood samples were collected from the simulated high altitude divers, before entrance into the chamber, during stay at high altitudes, upon completion of the dive and after surfacing from the chamber, for the detection of MDA, SOD, GSH-Px levels, the activities of antioxidases and catalase （CAT） , and total anti-oxidative capacity （TAOC）. Results The levels of MDA fluctuated within the range of （ 3.31±1.35 ）-（6.98±3.52）μmol/L. The activities of antioxidases were within the normal range. MDA levels and GSH-Px activities were directly correlated with the oxygen partial pressure and exposure duration. In the hyperoxie and hypoxic alternating environments, EPO levels were negatively correlated with the activities of SOD and CAT. Conclusions No oxidative damage induced by hyperoxic and hypoxic alternating environments could be detected in the 4 divers in the simulated heliox dive.

关 键 词：高原环境 氦氧潜水 氧化损伤 丙二醛 抗氧化酶 

分 类 号：R84[医药卫生—航空、航天与航海医学]