高压氧对创伤性脑损伤大鼠神经行为和突触素表达的影响  

Effects of hyperbaric oxygen on neurological behavior and synaptophysin expression in rats with traumatic brain injury

作　　者：刘萍[1] 孙洁[1] 李冰沁[1] 但齐琴[2] 王廷华[2,3] 凌斌[1] 

机构地区：[1]昆明医学院第四附属医院,昆明650021 [2]四川大学附属华西医院神经疾病研究室 [3]昆明医学学院神经科学研究所,昆明650021

出　　处：《中华航海医学与高气压医学杂志》2012年第3期167-170,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：云南省自然科学基金资助项目

摘　　要：目的研究高压氧治疗对脑创伤大鼠神经行为和突触素表达的影响。方法24只SpragueDawley（sD）大鼠按数字表法随机分为3组，即假手术组、脑创伤组（采用50g重锤30ca高度自由落体撞击制备大鼠运动皮质区脑损伤模型）和高压氧治疗组[脑创伤＋高压氧治疗（每天1次，连续治疗7d）]，每组8只。术后13d对各组大鼠进行神经功能缺损（neurologicalseverityscoFe，NSS）评分，观察动物运动和平衡功能缺损及改进情况；免疫组织化学方法检测各组脑组织突触素的表达情况。结果大鼠脑创伤后出现不同程度抽搐、瘫痪和平衡功能缺失。NSS评分结果显示：与假手术组（0．31±0．11）比较，脑创伤组NSS评分（4．11±0．50）明显升高，差异有统计学意义（P〈0．05）；高压氧治疗组（3．07±0．45）较脑创伤组NSS评分明显减少，差异有统计学意义（P〈0．05）。免疫组化染色结果显示：脑创伤组脑组织突触素阳性表达数量（71±10）较假手术组（504-11）明显增加，差异有统计学意义（P〈0．05）；高压氧治疗组突触素阳性表达数量（894±13）较脑创伤组明显增加，差异有统计学意义（P〈0．05）。结论高压氧治疗能促进脑创伤大鼠神经功能恢复，其作用机制可能与突触素表达增加有关。Objective To study the effects of hyperbaric oxygen （HBO） on neurological behavior and synaptophysin expression in rats with traumatic brain injury （TBI）. Methods Twenty-four Sprague Dawley （SD） rats were randomly divided into 3 groups： the sham surgery group （the control group）, the TBI group （the animal model was developed with the 40 g weight-free-drop from a height of 30 cm）, and the HBO treatment group （TBI ＋ HBO treatment, once a day for 7 successive days ） , each consisting of 8 animals. Neurological severity score （NSS） evaluation was made to determine the disorder and improvement of neurological functions of all the animal groups, 13 days after surgery. Immunocytochemistry was performed to detect the expression of synaptophysin in the brain tissues of all the animal groups. Statistical analysis was made with the single-factor t test. Results The affected animals displayed convulsion,paralysis and disequilibrium to various extents, following TBI. Results showed that NSS scores of the TBI group elevated obviously （4.11 -＋ 0.50）, with statistical significance, when compared with those of the sham surgery group （0.31±0.11 ） （ P 〈O. 05）. Results of immunocytochemistry indicated that there was a positive expression of synaptophysin in the TBI group, with a significant increase in the amount of the expression （71±10） , when compared with that of the sham surgery group （50±11 ）, with statistical significance （ P 〈 0, 05 ）. For the HBO treatment group, NSS scores （3.07±0.45） decreased considerably, when compared with those of the TBI group, with statistical significance （P 〈 0.05）. The amount of synaptophysin expression in the HBO group（89±13 ） was significantly higher than that of the TBI group, with statistical significance （P 〈 O. 05 ）. Conclusions HBO treatment could promote recovery of neurological functions of the rats with TBI and the increased expression of synaptophysin might be involved in its mechanism.

关 键 词：高压氧 创伤性脑损伤 大鼠 神经功能缺损评分 突触素 

分 类 号：R651.15[医药卫生—外科学]