铜绿假单胞菌耐氨基糖苷类相关耐药基因的检测  

Tests on the correlated resistant genes of Pseudomonas aerugionsa to Aminoglycosides

作　　者：屈艳[1] 张崇[2] 张春晓[3] 

机构地区：[1]新乡医学院微生物学教研室,河南新乡453003 [2]新乡医学院药学院,河南新乡453003 [3]新乡医学院第一附属医院结核内科,河南卫辉453100

出　　处：《新乡医学院学报》2012年第7期502-504,共3页Journal of Xinxiang Medical University

摘　　要：目的了解对氨基糖苷类抗生素耐药的铜绿假单胞菌氨基糖苷类修饰酶(AMEs)基因和甲酰化酶基因的存在情况。方法用聚合酶链反应(PCR)测定临床分离的170株对氨基糖苷类抗生素耐药的铜绿假单胞菌5种主要的AMEs基因和2种甲酰化酶基因。结果铜绿假单胞菌的AMEs基因携带率较高(94.71%),其修饰酶基因检出率分别为aac(3)-Ⅱ 77.6%、aac(6')-Ⅰ 41.2%、ant(3″)-Ⅰ 40.0%、aph(3')-Ⅵ 21.8%、aac(3)-Ⅰ 0.0%,同时携带2种或2种以上AMEs基因的菌株占63.53%,甲酰化酶基因检出率分别为rmtD 1.8%、rmtA 0.0%。结论铜绿假单胞菌中AMEs基因携带率较高,同一菌株携带多个不同的AMEs基因现象较多;甲酰化酶基因携带率极低。Objective To study the existing situation of aminoglycoside modifying enzyme（AMEs）gene and methylation gene of aminoglycoside antibiotics resistant Pseudomonas aerugionsa.Methods One hundred and seventy strains of aminoglycoside antibiotics resistant Pseudomonas aerugionsa were separated,meanwhile,5 main kinds of AMEs gene and 2 methylation genes were detected by polymerase chain reaction（PCR）.Results The AMEs gene carrying rate of Pseudomonas aerugionsa was high（94.71%）,and the detection rates of its modification genes were aac（3）-Ⅱ 77.6%,aac（6′）-Ⅰ 41.2%,ant（3″）-Ⅰ 40.0%,aph（3′）-Ⅵ 21.8%,aac（3）-Ⅰ 0.0%,resectively.The isolates rate that simultaneously carrying two or more genetypes was 63.53%,and the detection rates of methylation genes were rmtD 1.8%,rmtA 0.0%.Conclusion The Pseudomonas aerugionsa that carrying the AMEs gene has a high level,and the genetype is multiple;but the carrying rate of methylation is very low.

关 键 词：铜绿假单胞菌 氨基糖苷类修饰酶 甲酰化酶 基因型 耐药 

分 类 号：R915[医药卫生—微生物与生化药学]