miR-29a高表达促进大鼠骨髓间充质干细胞凋亡  

High Expression of miR-29a Promote Apoptosis in Rat Bone Marrow Mesenchymal Stem Cells

作　　者：段萍[1,2] 刘俊玲[1] 刘永敏[1] 王若愚[3] 韩雪飞[1,2] 鄢文海[1,2] 邢莹[2,4] 

机构地区：[1]郑州大学基础医学院 [2]郑州大学医学院干细胞研究中心 [3]郑州大学临床医学系,2008级本科生河南郑州450052 [4]新乡医学院,河南新乡453003

出　　处：《河南职工医学院学报》2012年第3期263-266,共4页Journal of Henan Medical College For Staff and Workers

摘　　要：目的检测外源性高表达miR-29a对骨髓间充质干细胞(MSCs)凋亡的影响。方法体外分离和培养大鼠MSCs,慢病毒介导外源性miR-29a在第四代MSCs中高表达,荧光定量PCR检测miR-29a表达,流式细胞术检测miR-29a高表达对MSCs凋亡的影响。结果慢病毒感染MSCs后,荧光定量PCR显示miR-29a表达显著增高,流式细胞术检测miR-29a高表达组和对照组(感染病毒空载体)的细胞凋亡率分别为(35.22±6.72)%和(14.21±3.27)%,坏死率分别为(20.01±5.68)%和(9.74±4.23)%,显示miR-29a高表达促进MSCs凋亡(P<0.05)。结论 miR-29a高表达促进MSCs凋亡。Objective To study the effect of miR-29a on cell apoptosis in bone marrow mesenchymal stem cells （MSCs）. Methods Rat MSCs were separated and cultured in vitro. Lentivirus-miR-29a was used to increase the exogenous miR-29a expression in the fourth passage MSCs. The expression of miR- 29a was detected by fluorimetric determination PCR and apoptosis was tested by flow cytometry. Results The expression of miR-29a increased significantly in lentivirus-transfected MSCs. The apoptosis rate in miR-29a expression group and control （transfected by lentivirus blank vector） was （35.22±6.72）% and （14.21±3.27）%, and the necrosis rate was （20.01±5.68）% and （9.74±4.23）%, separate- ly. Conclusion The high expression of miR-29a promotes apoptosis of MSCs.

关 键 词：miR-29a 骨髓间充质干细胞 慢病毒 凋亡 

分 类 号：R68[医药卫生—骨科学]