24份芦笋种质的ISSR分析  

ISSR Analysis of 24 Asparagus Germplasms

作　　者：代真真[1,2] 江程记[1,2] 杨峰[1,2] 高建明[1] 陈河龙[1] 郑金龙[3] 易克贤[3] 

机构地区：[1]海南大学农学院,海南儋州571737 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室,海南海口571101 [3]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《热带农业科学》2012年第5期35-40,共6页Chinese Journal of Tropical Agriculture

基　　金：公益性行业(农业)科研专项经费项目(No.201003074)

摘　　要：对收集的24个芦笋品种进行ISSR分析。结果表明:共计获得98个扩增位点,多态性位点74个,占总扩增位点数的75.61%。每条引物平均扩增位点数为10个。采用NTSYS2.1软件进行相似系数分析,建立聚类图,在阈值为0.771可以把中国、日本、意大利和美国等4个国家的品种区分开来,但是荷兰和美国的品种却聚为一类。主成分分析结果与相似系数分析结果基本上一致。Twenty-four asparagus germplasms as experimental materials were analyzed by using inter-simple sequence repeat （ISSR）, and the results showed that 98 fragments of DNA were amplified with 10 primers by using ISSR-PCR, among which 74 （75.61%） fragments were polymorphic. Each primer amplified 10 fragments of DNA. The genetic similarity coefficient was analyzed, and a tree diagram constructed by using UPGMA algorithm. According to this tree diagram four countries varieties form China, Japan, Italy and America were distinguished, but the Netherlands and the USA cultivars were constructed one group; and the results of the principal component analysis and the similarity coefficient analysis are basically consistent.

关 键 词：芦笋 ISSR 聚类分析 PCA 亲缘关系 

分 类 号：S644.6[农业科学—蔬菜学]