豇豆轮纹病病原鉴定及其室内药剂筛选  

Pathogen Identification of Cowpea Ring Spot and Screening of Fungicides

作　　者：王爽[1] 孔祥义[2] 林春花[3] 李博勋[3] 黄贵修[3] 

机构地区：[1]海南大学农学院,海南海口570228 [2]三亚市南繁科学技术研究院,海南三亚572000 [3]中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海南儋州571737

出　　处：《热带农业科学》2012年第5期61-65,共5页Chinese Journal of Tropical Agriculture

基　　金：海南省星火产业带专项资金项目"热带地区设施栽培豇豆新品种示范推广";国家科技支撑项目"主要冬种北运蔬菜高效栽培技术研究与示范"(No.2009BADA2B04)

摘　　要：从海南三亚豇豆设施棚内采集具典型轮纹病病斑的豇豆叶片,采用组织分离法分离到1株致病菌,根据病害症状特点、病原菌形态特征、培养性状、ITS序列分析,鉴定豇豆轮纹病的病原菌为多主棒孢霉[Corynespora cassiicola(Berk.&Curst.)Wei];利用生长速率法,对12种药剂进行室内筛选,结果表明:10%苯醚甲环唑(WP,可湿性粉剂)和50%异菌脲(WP)对病原菌具有强烈抑制作用,其EC50分别为2.709 46和9.093 27mg/L,而75%百菌清(WP)和50%多菌灵(WP)抑制效果较差,EC50分别为792.261和1 329.89 mg/L。A pathogen was isolated from diseased lesions on the leaves of cowpea in the facilities plantation bases of Sanya. According to the symptoms, morphological characteristics, culture characteristics and ITS sequence, the pathogen was identified as Corynespora cassiicola （ Berk. ＆ Curst.） Wei. Screening tests of 12 fungicides indicated that 10% Difenoconazole WP and 50% Iprodione WP showed good inhibition against C. cassiicola, the ECso value were 2.709 46 mg/L and 9.093 27 mg/L respectively, whereas 75% Chlorothalonil WP and 50% Carbendazim WP had poor inhibition effect. The ECs0 value were 792.261 mg/L and 1 329.89 mg/L respectively.

关 键 词：豇豆 轮纹病 病原 杀菌剂 筛选 

分 类 号：S643.4[农业科学—蔬菜学]