Formestane、17β-estradiol诱导鸡、鹌鹑胚胎性反转后bcl-2、p53基因的表达差异性研究  

On the Expression Differences between bcl-2 and p53 Genes after Sex Reverse in Chicken,Quail and Hybrid Embryos Induced by Formestane and 17β-estradiol

作　　者：陈丹盈[1] 梁耀伟[1] 赵宗胜[1] 冯欣璐[1] 班谦[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2012年第2期162-166,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30660125)

摘　　要：利用外源激素Formestane和17β-estradiol对鸡、鹌鹑及其属间杂交种胚胎进行了性反转实验,并分别采集了72、96、120、144、168、192h等6个时间点活胚,运用荧光定量PCR法分析了外源激素处理后的不同种胚胎不同时期的bcl-2和p53基因的表达量差异和变化。结果表明:正常鸡和鹌鹑胚种内异性间相比bcl-2和p53基因表达量差异不显著,杂交种胚分别与鸡胚、鹌鹑胚相比,2个基因表达量均差异显著(P<0.05);72h雌、雄鸡和鹌鹑的胚胎p53/bcl-2表达均出现低谷,此时p53表达同样降至低谷,推测胚胎大批死亡可能是在这个时间段;注射Formestane和17β-estradiol的鸡、鹌鹑胚bcl-2和p53基因表达量分别与未经处理的雌雄鸡胚、雌雄鹌鹑胚比较,差异显著(P<0.05)。而经外源激素处理的杂交种胚胎培养到72h检测全部死亡。In this study, using exogenous hormones Formestane and 17β-estradiol, sex reversal experiments were conducted among chicken, quail and their hybrids embryos, and live embryos of 6 time points （72,96,120,144,168 and 192 h） were then sampled correspondingly. By quantitative PCR,we analyzed expressional differences of bcl-2 and p53 genes of different embryos at different stages after hormone treatment. The results revealed that bcl-2 and p53 gene expression showed no significant difference between normal chicken and quail embryos within heterosexual groups, but compared with chicken and quail embryos,there was significantly difference in hybrid embryos（P〈0.05）;At 72 h, p53/bcl-2 of male and female embryos of chicken and quail both appeared low gene expression,and meantime, the p53 mRNA expressional quantity fell to relative low point, which indicated that the most embryos＇ death might happen at this period;gene expressions of bcl-2 and p53 of the chicken and quail embryos injected with Formestane and 17-estradiol were quite different from normal chicken and quail embryos（P＇〈0. 05）. But hybrid embryos were all dead at 72 h after they were injected exogenous hormones.

关 键 词：鸡 鹌鹑 BCL-2 P53 性反转 

分 类 号：Q954.4[生物学—动物学] S839[农业科学—畜牧学]