天麻素对脑缺血再灌注损伤大鼠神经细胞凋亡的抑制作用  

Inhibitory action of gastrodine on the neuronal apoptosis after focal cerebral ischemia reperfusion

作　　者：聂晶[1,2] 杜亮[2] 陆远富[1,2] 石京山[1,2] 

机构地区：[1]遵义医学院药理学教研室,贵州遵义563000 [2]贵州省基础药理重点实验室,贵州遵义563000

出　　处：《重庆医学》2012年第18期1841-1842,I0002,共3页Chongqing medicine

基　　金：遵义市红花岗区科技局科学技术项目[遵红科合社字(2007)13号]

摘　　要：目的探讨天麻素对脑缺血再灌注损伤大鼠的保护作用机制。方法采用线栓法建立大鼠局灶性脑缺血再灌注损伤模型,缺血2h,再灌注24h后取大鼠大脑组织进行HE染色观察病理改变,TUNEL法计数凋亡细胞,Real time RT-PCR检测胱天蛋白酶3(casepase-3)mRNA的表达。结果天麻素能明显改善中动脉闭塞所致局灶性脑缺血再灌注损伤大鼠脑组织病理变化,减少细胞凋亡(P<0.01),降低caspase-3mRNA的表达(P<0.05)。结论天麻素可通过下调caspase-3mRNA的表达减少大脑神经细胞凋亡,对缺血再灌注损伤大鼠脑组织发挥保护作用。Objective To investigate the potential mechanisms of gastrodine on the focal cerebral ischemic reperfusion damage in rats. Methods The SD rats were administered with gastrodine by gavage once a day for 7 days. The animals were then subjected to right-middle cerebral artery occlusion for 2 h and reperfusion for 24 h. Brain tissues were stained with hematoxylin-eosin. The num- ber of neuronal apoptosis was assessed by terminal oxynucleotidyl transferase mediated dUTP biotin nick end labeling（TUNEL）. The mRNA level of caspase-3 was detected by Real time RT-PCR. Results Compared with the model group,gastrodine significant- ly attenuated the apoptosis and the expression of caspase-3 of rats（P^0.05）. Conclusion Gastrodine can improve the brain injury induced by focal cerebral ischemia-reperfusion in rats. The mechanisms appear to be partly due to the inhibition of apoptosis through the suppression of caspase-3 expression.

关 键 词：天麻属 脑缺血 再灌注损伤 细胞凋亡 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R285.5[医药卫生—中药学]