实验性自身免疫性肌炎动物模型的制作  

Experimental autoimmune myositis model induced by pure myosin of rabbit

作　　者：段枫[1] 

机构地区：[1]中国人民解放军海军总医院神经内科,北京100048

出　　处：《重庆医学》2012年第18期1843-1844,共2页Chongqing medicine

摘　　要：目的探讨实验性自身免疫性肌炎(EAM)动物模型制作方法。方法实验动物选用健康雌性英国短毛豚鼠20只,分为正常组5只、佐剂对照组5只、EAM组10只。正常组不予任何处理;佐剂对照组采用完全弗氏佐剂(CFA)0.25mL加等量PBS;EAM组采用CFA 0.25mL加等体积浓度为10mg/L的兔纯化肌球蛋白悬液,分别于0、7、14、21d进行分次皮下注射,并在0、7d同时联合腹腔注射百日咳杆菌原液。评价实验动物的临床症状、血肌酶及病理改变。结果正常组及佐剂对照组动物的临床症状、血肌酶及病理改变未见异常。EAM组动物血肌酶升高,临床症状与病理改变与人类多发性肌炎类似,模型成功率为80%。结论应用纯化肌球蛋白制作实验性自身免疫性肌炎动物模型死亡率低,成功率高,为研究人类发性炎性肌病发病机理和治疗提供较好的工具,肌球蛋白可能是诱发自身免疫反应的候选成分之一。Objective To explored the method of experimental autoimmune myositis animal model. Methods Twenty female guinea pigs were randomly assigned into 3 groups. No treatment was given to the animals in normal group（n= 5）. The animals in the adjuvant control group（n=5） were subcutaneous injected with complete Freund＇s adjuvant（CFA） and equal amount of PBS. The animals in experimental autoimmune myositis（EAM） group（n= 10） received CFA plus equal volume rabbit purified myosin suspension（10 mg/L） in at day 0,7,14,21 respectively, combined with intraperitoneal injection of Bordetella pertussis dope（live bacteria count~ 4.0 X 10l~） at day 0,7. The clinical symptoms of experimental animals, blood creatine kinase（CK）and pathological changes were evaluated. Results The blood CK of the guinea pigs in normal and control group were normal, but abnormal elevated in EAM group. The clinical findings and pathologic changes of the guinea pigs in normal and control group were normal, and in EAM group were similar to the human% in idiopathic inflammatory myopathy. The model success rate was 80%. Conclusion Ex- perimental autoimmune myositis model induced by pure myosin, which was with low mortality and high success rate, provided better tools for the study of the pathogenesis and treatment in inflammatory myopathy. Myosin might be a probable antigen.

关 键 词：实验性自身免疫性肌炎 肌球蛋白 完全弗氏佐剂 百日咳杆菌 

分 类 号：R593.2[医药卫生—内科学]