交叉响应传感器鉴别β受体激动剂及其混合体系  

Cross-reactive sensor arrays for the determination of five β-agonists and their mixture

作　　者：侯长军[1] 戴斌[1] 霍丹群[1] 张玉婵[1] 董家乐[1] 秦辉[1] 李娟[1] 莫炼[1] 宋坤[1] 

机构地区：[1]重庆大学生物工程学院生物"流变科学与技术"教育部重点实验室,重庆400030

出　　处：《分析试验室》2012年第7期1-4,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(31171684;31101284);国家"863"项目(2006AA04Z349);中央高校研究生创新基金(CDJXS102300);重庆市科委攻关项目(2008AB2024)资助

摘　　要：开发了一种鉴别β受体激动剂的新型阵列传感器。该传感器由8种传感物质构成,使用96孔板酶标仪采集响应数据,结合主成分分析(PCA)、分层聚类分析(HCA)、判别分析(LDA)等模式识别方法进行数据处理,对5类β受体激动剂及其混合物进行检测。PCA结果表明,该传感器主要是基于空间结构以及氢键作用实现对β受体激动剂的识别;HCA结果显示,93个分析样本归类正确;LDA结果显示,该传感器对于β受体激动剂识别的准确率达98.9%。本方法在β受体激动剂的检测中有潜在应用价值。A novel sensor consisting of 8 chemical dyes was developed for the determination of β-agonists.The data was recorded by a 96-well plate reader,then be analyzed with statistical and stoichiometric methods,including principal component analysis（PCA）,hierarchical clustering analysis（HCA） and linear discriminant analysis（LDA）.PCA suggests that the major mechanism of identification is molecular shape and hydrogen bond HCA demonstrated that five β-agonists and their mixtures could be well separated.LDA showed the classification accuracy of identification was 98.9%.These results indicate that the array sensor can be used for the rapid identification of β-agonists and their mixture.

关 键 词：Β受体激动剂 交叉响应 阵列传感器 

分 类 号：O657.1[理学—分析化学]