多粘类芽孢杆菌HY96-2产脂肽类抗真菌物质的研究  

Antifungal Lipopeptide Produced by Paenibacillus polymyxa HY96-2

作　　者：范磊[1] 张道敬[1] 刘振华[1] 陶黎明[2] 罗远婵[1] 魏鸿刚[1] 李淑兰 李元广[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室 [2]华东理工大学药学院 [3]上海泽元海洋生物技术有限公司,上海200237

出　　处：《天然产物研究与开发》2012年第6期729-735,共7页Natural Product Research and Development

基　　金：国家农业科技成果转化资金项目(2007GB2C000101);国家863项目(2006AA10A211);上海市重点学科建设项目(B505)

摘　　要：对一株已经商业化的可防治植物枯萎病的生防菌-多粘类芽孢杆菌HY96-2发酵液中抗真菌活性物质进行了分离纯化,采用酸化、正丁醇萃取、乙酸乙酯沉淀、硅胶柱层析、Sephadex LH-20柱层析、高效液相色谱(HPLC)等方法分离得到了一个抗真菌活性化合物6B,经NMR、MS、MS/MS等光谱学方法鉴定其为Fusaricidin A。琼脂扩散法抑菌试验结果表明,6B对西瓜枯萎病菌、水稻纹枯病菌、灰霉病菌等15种植物病原真菌的最小抑菌浓度为12.5～50μg/mL。盆栽实验结果表明,6B对黄瓜灰霉病具有明显的防治效果,当6B的浓度达到250μg/mL时,防治效果高达95%。Isolation and purification of antifungal substance from Paenibacillus polymyxa HY96-2,a commercial biocontrol strain used in control of fusarium wilt disease,was studied in this paper.By means of acidification,n-BuOH extraction,ethyl acetate precipitation,silica gel column chromatography,sephadex LH-20 column chromatography and HPLC preparation,6B was isolated and purified.Its structure was elucidated as fusaricidin A by means of spectroscopic analysis including NMR,MS and ESI-MS/MS.Results of two-fold serial agar dilution method showed that the minimum inhibition concentrations of 6B against 15 phytopathogenic fungi were 12.5-50 μg/mL.Greenhouse results showed that 6B had better control effect on Botrytis cinerea of cucumber.The control effect could reach 95% when its concentration was set at 250 μg/mL.

关 键 词：多粘类芽孢杆菌 抗真菌活性 分离纯化 结构鉴定 Fusaricidin A 

分 类 号：TQ458[化学工程—农药化工]