超高效液相色谱法同时测定茯苓中去氢土莫酸等6种活性成分的含量  

UPLC Determination of determine dehydrotumulosic acid etc. Six Active Components in Poria

作　　者：张靓琦[1] 贾英[1] 罗洁[1] 崔思娇[1] 黄健[1] 毕开顺[2] 

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]沈阳药科大学药学院,沈阳110016

出　　处：《中国药学杂志》2012年第13期1080-1083,共4页Chinese Pharmaceutical Journal

基　　金：中药制药过程新技术国家重点实验室开发基金课题资助项目(SKL2010M0201)

摘　　要：目的建立同时测定茯苓中去氢土莫酸、土莫酸、猪苓酸C、3-表去氢土莫酸、去氢茯苓酸、茯苓酸的超高效液相色谱。方法采用ACQUITY UPLC,HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相为乙腈-0.05%的磷酸水溶液梯度洗脱,检测波长采用210 nm。结果去氢土莫酸、土莫酸、猪苓酸C、3-表去氢土莫酸、去氢茯苓酸和茯苓酸质量浓度与峰面积分别在5.400～108.0,2.040～40.80,5.020～100.4,2.120～42.40,5.060～101.2和5.100～102.0μg.mL-1内呈良好的线性关系。平均回收率去氢土莫酸为98.0%,RSD为2.9%;土莫酸为99.0%,RSD为2.8%;猪苓酸C为101.5%,RSD为2.5%;3-表去氢土莫酸为97.7%,RSD为2.7%;去氢茯苓酸为101.5%,RSD为2.1%;茯苓酸为99.6%,RSD为1.1%。结论该方法快速、准确,分离度好,可用于同时测定茯苓中多种三萜酸的含量。OBJECTIVE To establish an analytical method to determine dehydrotumulosic acid, tumulosic acid, polyporenic acid C, 3-epi-dehydrotumulosic acid, dehydropachymie acid and pachymic acid in poria quickly and accurately. METHODS An UPLC method was established on an HSS T3 Column （2. 1 mm × 100 mm, 1.8 μm）. The mobile phase consisted of water（ containing 0. 05% phosphoric acid） and aeetonitrile in gradient elution mode, and the detection wavelength was set at 210 nm. RESULTS The standard curves of dehydrotumulosie acid, tumulosic acid, polyporenic acid C, 3-epi-dehydrotumulosic acid, dehydropachymic acid, and pachymic acid showed good linearity in 5.400 - 108.0, 2. 040 -40. 80, 5. 020 - 100. 4, 2. 120 -42. 40, 5. 060 - 101.2 and 5. 100 - 102.0 μg mL-1 , respectively and the average recoveries were 98.0% with RSD of 2. 9% for dehydrotumulosic acid, 99. 0% with RSD of 2. 8% for tumulosic acid, 101.5% with RSD of 2. 5% for polyporenic acid C, 97.7% with RSD of 2. 7% for 3-epi-dehydrotumulosic acid, 101.5% with RSD of 2. 1% for dehydropachymic acid and 99. 6% with RSD of 1.1% for pachymic acid, respectively. CONCLUSION The method is quick, accurate, and can be used to determine multiple triterpenoid acids in Poria simultaneously.

关 键 词：茯苓 去氢土莫酸 土莫酸 猪苓酸C 3-表去氢土莫酸 去氢茯苓酸 茯苓酸 超高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]