双抗体夹心ELISA检测人胎儿血红蛋白体系的建立  

Establishment of a sandwich-ELISA system for determination of human fetal hemoglobin

作　　者：李雪丽[1] 文李艳[1] 冯善伟 钟梅[3] 刘艳君[1] 富宁[1] 

机构地区：[1]南方医科大学基础医学院免疫教研室,广东广州510515 [2]广州市人口和计划生育科学研究所,广东广州510410 [3]南方医科大学南方医院,广东广州510515

出　　处：《中国实验诊断学》2012年第6期997-1000,共4页Chinese Journal of Laboratory Diagnosis

基　　金：广东省科技项目2007B031512001

摘　　要：目的建立一种定量检测人胎儿血红蛋白(HbF)的双抗体夹心ELISA方法。方法用抗人HbF单抗作为捕获抗体,加入待测抗原,检测抗体为兔抗人HbF多克隆抗体,最后加入辣根过氧化物酶标记的羊抗兔IgG。结果本检测体系灵敏度约为0.039μg/ml,测量范围0.039-24.66μg/ml,特异性强,重复性好,100份血标本的检测结果与高效液相色谱法结果符合率高。结论建立了一种可用于检测人外周血HbF的双抗体夹心ELISA方法,有望为临床上诊断某些与HbF有关的疾病提供帮助。Objective To establish a sandwich ELISA for the determination of fetal hemoglobin （HbF） in human blood. Methods An anti-HbF monoclonal antibody was coated on polystyrene microtiter plates followed by blocking with 5% defat milk,then samples for detection were added to the wells,followed by anti-HbF polyclonal antibody prepared from rabbit;at last＇, addition of HRP-goat anti-rabbit IgG and conventionhl color development were performed. Resuits This sandwich ELISA system can determine HbF specifically,the sensitivity for HbF is 0. 039μg/ml,the range of detection is 0. 039-24.66μg/ml. Conclusion This HbF ELISAis applicable for research and observation in clinical.

关 键 词：胎儿血红蛋白 双抗体夹心ELISA 多克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]