人参皂苷Rb1对Aβ淀粉样蛋白诱导大鼠海马神经元损伤的保护作用  

Protective effect of ginsenoside Rb1 on hippocampal neuron injury after treated by Aβ-amyloid protein in rats

作　　者：王洪财[1] 蒋玉萌[1] 赵雪[1] 王宁[1] 高迪[2] 关铭[2] 张惊宇[1] 杨子超[1] 

机构地区：[1]哈尔滨医科大学附属第四医院神经内科,黑龙江哈尔滨150001 [2]吉林大学药学院药物分析教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2012年第3期447-450,F0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家科技重大专项"重大新药创制"项目资助课题(2009ZX09502-011)

摘　　要：目的:研究人参皂苷Rb1对Aβ淀粉样蛋白诱导大鼠海马神经元损伤的影响,为其临床应用提供理论依据。方法:体外原代培养的SD大鼠海马神经元经Aβ淀粉样蛋白损伤建立细胞模型,实验分为正常对照组、Aβ淀粉样蛋白诱导损伤对照组(损伤对照组)、Rb1低剂量组(10mg.L-1)、Rb1中剂量组(50mg.L-1)及Rb1高剂量组(100mg.L-1),应用MTT法检测海马神经元生长增殖活力,TUNEL法检测神经元凋亡情况,化学发光法检测海马神经元内caspase-3活性。结果:MTT法检测结果显示,与正常对照组比较,损伤对照组和Rb1低、中及高剂量组神经元生存率均有不同程度的下降(P<0.05),损伤对照组下降最明显;与损伤对照组比较,Rb1中、高剂量组神经元生存率明显升高,差异有统计学意义(P<0.05)。TUNEL法检测结果显示,与正常对照组比较,损伤对照组、Rb1低、中剂量组的阳性神经元数量明显增加,其中损伤对照组增加最明显;损伤对照组与Rb1高剂量组比较差异有统计学意义(P<0.05)。损伤对照组caspase-3活性与Rb1高剂量组比较差异有统计学意义(P<0.05)。结论:人参皂苷Rb1对Aβ淀粉样蛋白诱导性神经元损伤具有保护作用,并通过调控细胞凋亡实现。Objective To explore the effect of ginsenoside Rb1 on hippocampal neuron injury after treated by Aβ-amyloid protein in rats and provide theoretical basis for its clinic application.Methods The cultured hippocampal neurons from newborn SD rats were divided into normal control group,hippocampal neurons treated with Aβ-amyloid protein group（injury control group）,low dose（10 mg·L-1） Rb1 group,middle dose（50 mg·L-1） Rb1 group,and high dose（100 mg·L-1） Rb1 group.Then the survival rates of neurons were detected by MTT colorimetry and the apoptosis of neurons were detected by TUNEL staining,and the caspase-3 activities of neurons were detected by immunohistochemistry method.Results The MTT results showed that the survial rates of hippocampal neurons were decreased in injury control group,10 mg·L-1 Rb1 group,50 mg·L-1 Rb1 group and 100 mg·L-1 Rb1 group compared with normal control group（P〈0.05）.Compared with injury control group,the survial rates in 50 mg·L-1 Rb1 group and 100 mg·L-1 Rb1 group were increased（P〈0.05）.The TUNEL results showed that compared with normal control group,the apoptosis of neurons in injury control group,10 mg·L-1 Rb1 group,50 mg·L-1 Rb1 group and 100 mg·L-1 Rb1 group were increased（P〈0.05）.The aproptotic rate of neurons in 100 mg·L-1 Rb1 group was higher than that in injury control group（P〈0.05）.The expression of caspase-3 in 100 mg·L-1 Rb1 group was lower than that in injury control group（P〈0.05）.Conclusion Ginsenosides Rb1 can protect hippocampal neurons against Aβ-amyloid protein injury,which is achieved through the regulation of apoptosis.

关 键 词：人参皂苷RB1 海马神经元 CASPASE-3 细胞凋亡 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]