成纤维细胞生长因子-21对体外诱导的非酒精性脂肪肝细胞模型脂代谢的影响  

Effect of fibroblast growth factor-21 on lipid metabolism of nonalcoholic fatty liver disease cell model induced in vitro

作　　者：刘敏[1] 孔祥鑫[1] 杨苹[1] 陈玉斌 李校堃[1] 王会岩[1,2] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]吉林医药学院检验系,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2012年第3期477-481,I0001,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(20116044)

摘　　要：目的:探讨成纤维细胞生长因子-21(FGF-21)对体外诱导的非酒精性脂肪肝(NAFLD)细胞模型脂代谢的影响及其可能的作用机制。方法:采用MTT法筛选医用脂肪乳注射液使用浓度和FGF-21作用浓度,将体外诱导的NAFLD细胞分为对照组、模型组、脱离脂肪乳组和FGF-21治疗组,光学显微镜观察油红O染色情况,全自动生化仪检测NAFLD细胞内甘油三酯(TG)水平,免疫细胞化学方法观察细胞内肉毒碱脂酰转移酶-1(CPT-1)、过氧化氢酶体增殖物激活受体γ(PPARγ)及固醇调节元件结合蛋白-1c(SREBP-1c)的表达。结果:MTT法筛选出0.1%医用脂肪乳注射液作为建立体外诱导NAFLD细胞模型的最佳诱导浓度,与正常肝细胞株HL7702比较,模型组油红O染色后细胞浆内有大量被染成橘红色的脂滴,细胞内TG水平显著升高(P<0.01),CPT-1蛋白表达减弱,PPARγ和SREBP-1c蛋白表达增加。MTT法筛选出0.5mg.L-1FGF-21作为观察该药物对NAFLD细胞模型影响的作用浓度,与模型组比较,FGF-21处理组细胞内TG水平显著降低(P<0.01),CPT-1蛋白表达增加,PPARγ和SREBP-1c蛋白表达减弱。结论:FGF-21处理能减轻NAFLD细胞中TG蓄积,并使HL7702细胞CPT-1蛋白表达增加,而PPARγ和SREBP-1c蛋白表达减少。Objective To study the effect of fibroblast growth factor-21（FGF-21） on the lipid metabolism of nonalcoholic fatty liver disease（NAFLD）cell model induced in vitro and its potential mechanism.Methods The concentrations of medical fat emulsion（MFE） and FGF-21 were selected by MTT assay.And the NAFLD cells were divided into control group,model group,leaving fat emulsion group and FGF-21 therapy group.Oil red O staining was used to observe lipid droplet under light microscope.The intracellular triacylglycerol（TG） levels in each group were observed by hol-automatic biochemistry.The expressions of carnitine palmitovl transferase-1（CPT-1）,peroxisome proliferator-activated receptor-gamma（PPARγ） and sterol regulatory element binding protein-1c（SREBP-1c） were determined by immunocytochemistry.Results The 0.1% medical fat emulsion（MFE） was chosen by MTT assay as the best concentration for the induction of NAFLD cells in vitro.The intracellular TG level of NAFLD cells was increased significantly compared with normal liver cells HL7702（P〈0.01）,and there was a large quantity of orange-red lipid droplet in cytoplasm of NAFLD cells;meanwhile,the expression of CPT-1 protein got lower,and the expressions of PPARγ and SREBP-1c proteins got higher.MTT assay showed the best management concentration of FGF-21 was 0.5 mg·L-1 for setting up NAFLD-effect model,the intracellular TG level in FGF-21 group was decreased significantly compared with model group（P〈0.01）;the expression level of CPT-1 protein was increased（P〈0.01）,and the expressions of PPARγ and SREBP-1c proteins were decreased in FGF-21 group.Conclusion FGF-21 is helpful to relieve accumnlation of TG in NAFLD cells,and it decrease the expressions of PPARγ and SREBP-1c protein and increase the expression of CPT-1 protein in HL7702 cells.

关 键 词：成纤维细胞生长因子-21 非酒精性脂肪肝病 医用脂肪乳注射液 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]