神经肽Y在杏仁中央核内对甜味觉的调节及其机制  

Regulation of neuropeptide Y on sweet taste in central nucleus of amygdala and its mechanism

作　　者：朱永香[1] 王倩[1] 王爽[2] 于玮[1] 南瑛[1] 曹健[1] 

机构地区：[1]西安医学院生理学教研室,陕西西安710021 [2]西安医学院病理生理学教研室,陕西西安710021

出　　处：《吉林大学学报（医学版）》2012年第3期512-516,I0002,共6页Journal of Jilin University：Medicine Edition

基　　金：西安医学院校级科研基金资助课题(2007QN05)

摘　　要：目的:观察神经肽Y(NPY)在杏仁中央核(CeA)内对甜味觉信息的调节方式,探讨其可能的作用机制。方法:选择58只雄性SD大鼠,将其中10只随机分为饮用蒸馏水组与饮用糖精溶液组,每组各5只,2h后取大鼠脑组织,应用免疫组织化学方法检测大鼠CeA内c-Fos及NPY表达的变化;将其余48只雄性SD大鼠行CeA内套管植入术,术后24只大鼠随机分为4组,2组CeA内注射NPY,2组CeA内注射生理盐水,2h后分别观测大鼠对糖精溶液或蒸馏水的摄入量;另24只术后大鼠随机分为4组,2组腹腔注射纳洛酮,2组腹腔注射生理盐水,20min后大鼠CeA内分别注射NPY或生理盐水,2h后分别观察大鼠对糖精溶液的摄入量。结果:大鼠摄入糖精溶液后CeA内NPY及c-Fos表达显著增加(P<0.05);大鼠CeA内注射NPY使糖精溶液摄入量明显增加(P<0.05);纳洛酮预处理使CeA内注射NPY后糖精溶液摄入量明显减少(P<0.05)。结论:NPY在CeA内参与了糖精甜味觉信息的调控,其机制可能与阿片系统有关。Objective To examine the regulation of neuropeptide Y（NPY） on sweet taste in the central nucleus of amygdala（CeA） and to explore its possible mechanism.Methods 58 male SD rats were chosen,and 10 rats of them were divided into drinking saccharin solution group（n=5） and drinking distilled water group（n=5）;after 2 h,the rat brain was obtained.The expressions of NPY and c-Fos in CeA were detected by immunohistochemical staining method.The other 48 rats were implanted cannula in CeA.The postoperative 24 rats were randomly divided into 4 groups,and the rats in 2 groups were injected with NPY and the rats in the other 2 groups were injected with saline in CeA,and the intake of saccharin solution and distilled water were detected 2 h later.The other postoperative 24 rats were randomly divided into 4 groups,and the rats in 2 groups were intraperitoneally injected with naloxone and the rats in the other 2 groups were intraperitoneally injected with saline,after 2 min,the rats were injected with NPY or saline in CeA,and the intake of saccharin solution were detected 2 h later.Results The NPY and c-Fos expressions in CeA were significantly increased after drinking saccharin solution（P〈0.05）.The intake of saccharin solution was significantly increased after injecting NPY in CeA（P〈0.05）;the pre-treatment of naloxone blocked the orexigenic effect of NPY（P〈0.05）.Conclusion NPY in CeA may play an important role in the regulation of sweet taste of saccharin solution,and the mechanism may be related to the opioid system.

关 键 词：神经肽Y 杏仁中央核 C-FOS表达 甜味觉 

分 类 号：R338.2[医药卫生—人体生理学]