Tumor suppressor gene NGX6 induces changes in protein expression profiles in colon cancer HT-29 cells  

作　　者：Yu Li Yuan Luo Xiaoyan Wang Shourong Shen Haibo Yu Jing Yang Zheng Su 

机构地区：[1]Department of Gastroenterology,Third Xiangya Hospital,Central South University,Changsha 410013,China [2]Department of General Surgery,Second Xiangya Hospital,Central South University,Changsha 410011,China [3]Cancer Research Institute,Central South University,Changsha 410078,China [4]Present address:Hunan Tumor Hospital,Changsha 410013,China

出　　处：《Acta Biochimica et Biophysica Sinica》2012年第7期584-590,共7页生物化学与生物物理学报（英文版）

摘　　要：Nasopharyngeal carcinoma-associated gene 6 （NGX6; syn. transmembrane protein 8B, TMEM8B） is a recently iden- tified tumor suppressor gene. The underlying mechanisms by which the gene inhibits tumor development are not completely known. To further understand the function of the gene＇s protein product NGX6, in the present study, we employed two-dimensional difference gel electrophor- esis to analyze the protein expression profiles of colon cancer HT-29 cells stably transfected with the gene NGX6. The differentially expressed proteins were selected and identified by matrix-assisted laser desorption/ionization coupled with time-of-flight tandem mass spectrometry. The results showed that 12 proteins were down-regulated and 4 were up-regulated in NGX6-transfected HT-29 cells, compared with vector-transfected HT-29 cells. The MS results were verified by western blot. Bioinformatic analysis showed that these proteins are involved in cell proliferation, metastasis, apoptosis, cytoskeletal structure, metabolism, and signal transduction, suggesting that NGX6 may inhibit colon cancer through the regulation of these biological processes.Nasopharyngeal carcinoma-associated gene 6 （NGX6; syn. transmembrane protein 8B, TMEM8B） is a recently iden- tified tumor suppressor gene. The underlying mechanisms by which the gene inhibits tumor development are not completely known. To further understand the function of the gene＇s protein product NGX6, in the present study, we employed two-dimensional difference gel electrophor- esis to analyze the protein expression profiles of colon cancer HT-29 cells stably transfected with the gene NGX6. The differentially expressed proteins were selected and identified by matrix-assisted laser desorption/ionization coupled with time-of-flight tandem mass spectrometry. The results showed that 12 proteins were down-regulated and 4 were up-regulated in NGX6-transfected HT-29 cells, compared with vector-transfected HT-29 cells. The MS results were verified by western blot. Bioinformatic analysis showed that these proteins are involved in cell proliferation, metastasis, apoptosis, cytoskeletal structure, metabolism, and signal transduction, suggesting that NGX6 may inhibit colon cancer through the regulation of these biological processes.

关 键 词：NGX6 colon cancer differential in-gelelectrophoresis mass spectrometry 

分 类 号：Q939.117[生物学—微生物学] Q785