黄绿青霉素对低硒低蛋白大鼠心肌损伤的生化检测  

Biochemical tests of rat cardiac injury induced by citreoviridin toxin in rats with joint deficiency of selenium and protein

作　　者：于佳男[1] 魏灿[1] 张鼎[1] 裴俊瑞[1] 李德安[1] 周令望[1] 冯红旗[1] 孙树秋[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心克山病防治研究所,150081

出　　处：《中国地方病学杂志》2012年第4期385-388,共4页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金（30872190）

摘　　要：目的观察黄绿青霉素（CIT）对低硒低蛋白大鼠心肌损伤在生化水平上的特点。方法48只4周龄Wistar雄性大鼠．2X2析因设计。按体质量随机分为4组：低硒低蛋白加CIT组、低硒低蛋白组、常硒常蛋白加CIT组、常硒常蛋白组。每组12只。分别采用低硒低蛋白和常硒常蛋白饲料喂养大鼠至第12周后，加毒素组大鼠饲料中加入8mg·kg^-1·d^-1 CIT喂养至第20周，再加量至10mg·kg^-1·d^-1 CIT继续喂养至22周，股动脉采血及取心脏。检测血清肌钙蛋白I（Tn—I）、白蛋白水平，肌酸激酶（CK）、谷胱甘肽过氧化物酶（GSH—Ix）以及心肌超氧化物歧化酶（SOD）活性，总抗氧化能力（T-AOC）。结果“硒＋蛋白”与CIT对大鼠终末体质量、白蛋白和Tn—I存在交互作用（F值分别为8．186、6．160、19．183，P均〈0．05），对12周大鼠体质量，22周血清GSH—Px、CK水平以及心肌SOD、T—AOC活性的影响不存在交互作用（F值分别为1．633、1．987、0．075、0．474、1．145，P均〉0．05）。在低硒低蛋白背景下，加CIT组血清白蛋白和Tn—I水平[（42．88±1．19）g／L，（668．6±55．8）ng／L]均低于无CIT组[（47．59±1．05）g／L，（989．3±49．2）ng／L，P均〈0．05]。“硒＋蛋白”对12周大鼠体质量、22周血清GSH—Px活性以及心肌SOD、T-AOC有影响（F值分别为96．860、58．086、4．475、25．485，P均〈0．05）。低硒低蛋白两组12周大鼠体质量[（186．33±7．89）、（197．83±7．89）g]均低于常硒常蛋白两组[（274．08-I-7．89）、（265．42±7．89）g，P均〈0．05]；低硒低蛋白两组[（317．5±102．6）、（296．9±90．5）U／L]血清GSH—Px活性均低于常硒常蛋白两组[（926．1±110．9）、（1181．7±85．9）U／L，P均〈0．05]；低硒低蛋白两组心肌SOD活性[（65．22±5．91）×106、（62．68±5．61）×106U／kg]均低于常硒常蛋白两组[（74．07±7．24）×1Objective To clarify the causative effect of citreoviridin toxins（CIT） as well as nutritional deficiency of selenium and protein on rat myocardial injury at biochemical level. Methods According to 2 × 2 factorial design, 48 healthy male Wistar rats aged g-week were randomly divided into four groups： exposed to CIT along with nutritional deficiency of selenium and protein, nutritional deficiency of selenium and protein, exposed to CIT and control groups. After the rats in each group began to be fed with selenium and protein deficiency and （or） adequate fodder for 3 months, 8 mg/kg body weight of CIT was fed daily to the rats in the two CIT toxin groups for two months. After that the CIT dose was raised up to 10 mg/kg body weight each day within the final 2 weeks. At the experimental endpoint, all the rats were sacrificed by femoral artery bleeding after ether anesthesia, and serum and heart specimens were collected for biochemical analysis by detecting serum Tn-I and albumin, serum activities of CK and GSH-Px, myocardial SOD and T-AOC. Results The interactions between ＂Se ＆ protein＂ and CIT in rat final body weight, serum albumin, and Tn-I was observed （F = 8.186, 6.160, 19.183, all P 〈 0.05）, whereas interactions in rat body weight of 12 weeks, serum GSH-Px, CK as well as myocardial SOD, T-AOC activity were not found （F = 1.633, 1.987, 0.075, 0.474, 1.145, all P 〉 0.05）. Under the nutritional deficiency of selenium and protein, serum albumin and Tn-I level in the groups with CIT toxin[ （42.88 ± 1.19） g/L, （668.6 ± 55.8） ng/L, respectively] were lower than that of the group without CIT toxin[ （47[59 ± 1.05）g/L, （989.3 ± 49.2）ng/L, respectively, all P 〈 0.05 ]. The main effects of selenium and protein on rat body weight of 12 weeks , serum GSH-Px, myocardial SOD and T-AOC were statistically significant between different groups （F = 96.860, 58.086, 4.475, 25.485, all P 〈 0.05）. Rat body weights of 12 weeks in the two groups of nutritional deficiency of seleniu

关 键 词：硒 黄绿青霉素 蛋白质 心肌 生物化学 

分 类 号：R542.3[医药卫生—心血管疾病]