结核性胸液中NK细胞的亚群及表型功能特征分析  

Phenotypic and biological characterization of human NK cells in pleural fluid cells

作　　者：付笑迎[1] 李丽[1] 李琴[1] 乔丹[1] 劳穗华[2] 杨滨燕[1] 吴长有[1] 

机构地区：[1]中山大学医学院免疫学研究所热带病防治研究教育部重点实验室,广州510080 [2]广州市胸科医院重症结核科中心ICU病区,510095

出　　处：《免疫学杂志》2012年第8期691-694,共4页Immunological Journal

基　　金：教育部博士研究生学术新人奖资助项目(50000-3191013)

摘　　要：目的探讨结核性胸液(PFC)中NK细胞的亚群分布及表型功能特征。方法以正常人外周血单个核细胞(PBMC)作对照,利用多种标记抗体进行表面和细胞内细胞毒效应分子染色,再利用流式细胞仪在单个细胞水平上分析结核性胸水中NK细胞亚群的异质性和生物学特征。结果 NK细胞可以分为CD56+CD16-、CD56+CD16+和CD56-CD16+三个亚群,与PBMC中的NK细胞相比,PFC中CD56+CD16-NK细胞亚群明显增加,而CD56+CD16+NK细胞亚群比例明显下降;结核性胸液中CD56+CD16-及CD56+CD16+NK细胞亚群表达较低水平的颗粒酶B,CD107a/b的比例在结核性胸液中3个NK细胞亚群中均有增加(P<0.05)。结核性胸液中NK细胞表达高水平表面抑制性受体NKG2A及表面活化性受体NKG2D。活化分子CD69及CD25在结核性胸水中NK细胞上的表达均有所增加(P<0.05)。结论结核性胸液中的NK细胞比例与PBMC相比发生变化,结核性胸液中的NK细胞表达低水平杀伤分子颗粒酶B但表达高水平活化分子CD69,提示NK细胞在结核感染中发挥其生物学功能。This paper aimed to characterize the phenotypic and biological properties of NK cells in human pleural fluid cells （PFCs）. Firstly, we isolated PFCs and PBMCs from pleural fluids and normal individuals, respectively. Then, surface markers and intracellular cytotoxic molecules of PFCs and PBMCs were stained with monoclonal antibodies and analyzed at a single cell level by flow cytometry. Our results showed that three distinct subpopulations （CD56＋CD16-, CD56＋CD16＋ and CD56-CD16＋） of NK cells from PFCs could be identified based on the expression of CD56 and CD16 molecules. The total number of NK cells was decreased and the subset of CD56＋CD16- NK cells in pleural fluid from tuberculosis patients was increased compared with PBMCs. The expression of granzyme B was significantly lower on subsets of CD56＋CD16＋ and CD56-CD16＋ NK cells from PFCs as compared with PBMCs, which was consistent with the higher level of CD107a/b on subsets of CD56＋CD16-, CD56＋CD16＋ and CD56-CD16＋ NK cells from PFCs （P〈O.05）. The expressions of NKG2A, NKG2D, CD69 and CD25 were markedly higher on subsets of CD56＋CD16＋ and CD56-CD16＋ NK ceils from PFCs as compared with PBMCs （P〈0.05）. In conclusion, the percentage of NK cells from PFCs is decreased compared with PBMCs; lower expression of granzyme B and higher expression of CD69 indicate that NK cells might fight against the tuberculosis infection.

关 键 词：NK细胞 结核性胸膜炎 颗粒酶B 抑制/活化性受体 活化分子 

分 类 号：R521.7[医药卫生—内科学]