抗环瓜氨酸肽抗体、葡萄糖6-磷酸异构酶及类风湿因子的检测及对类风湿关节炎的诊断价值  

Significance of rheumatoid factor,anti-CCP,and GPI assay in diagnosis of rheumatoid arthritis

作　　者：许福亮[1] 张霞[1] 王云海[1] 

机构地区：[1]山东潍坊医学院附属医院检验科山东省十二五高校重点实验室,261031

出　　处：《免疫学杂志》2012年第8期710-712,共3页Immunological Journal

摘　　要：目的为寻找敏感性高、特异性强的诊断类风湿关节炎(RA)的实验指标,选择抗环瓜氨酸肽(CCP)抗体、葡萄糖6-磷酸异构酶(GPI)和类风湿因子(RF)并探讨对RA的诊断价值。方法抗CCP抗体和GPI试验采用酶联免疫吸附(ELISA)法,RF用胶乳凝集法,检测了125例RA患者(RA组)、56例其他风湿性疾病患者(对照组)和36例健康体检者(正常组)血清中上述3者的浓度。结果抗CCP抗体、GPI及RF,在RA组分别是:(83.6±45.9)RU/ml、(2.8±2.3)μg/ml和(320.4±208.6)IU/ml;在对照组分别是:(36.2±15.3)RU/ml、(0.19±0.06)μg/ml和(36.3±12.5)IU/ml;在正常组分别是:(19.2±8.6)RU/ml、(0.16±0.08)μg/ml和(19.2±6.5)IU/ml。在RA组中,3者的敏感度分别是64.8%、73.6%和69.6%,特异性分别是92.0%、72.8%和74.4%。结论 RA患者血清抗CCP抗体、GPI和RF显著高于其他疾病患者(P<0.01)。抗CCP抗体和GPI有可能成为诊断RA的新指标、RA的血清标志物,对提高诊断RA的敏感性和特异性,具有良好的临床研究和推广价值。Rheumatoid arthritis （RA） is a common rheumatoid disease, which is easily misdiagnosed, and ideal diagnosis experiment is very few in early diagnosis. So it is necessary to look for the diagnostic experiment and clinic index with high sensitivity and specificity for the diagnosis of RA. In this essay, we analyzed anti-cyclic citrullinated peptide （CCP） antibody, 6-glucose phosphate isomerase （GPI） and rheumatoid factor （RF） for RA diagnose in different groups. All cases were divided into RA groups, control groups and normal groups. Anti-CCP antibody and GPI were determined by ELISA, while RF was determined by latex aggregation method in cases of all groups. Then the sensitivity and specificity were compared respectively. The concentrations of anti-CCP, GPI and RF in RA groups were all significantly different from the other groups （P〈 0.01）. In RA groups, the sensitivity of anti-CCP antibody, GPI and RF determination were respectively 64.8%, 73.6% and 69.6%, while the specificity were respectively 92.0%, 72.8% and 74.4%. Anti-CCP antibodies and GPI may become new targets of RA diagnosis with good clinical prospects.

关 键 词：抗CCP抗体 葡萄糖6-磷酸异构酶 类风湿因子 类风湿关节炎 

分 类 号：R593.22[医药卫生—内科学]