王氏管精子优选法的应用及效果  

Effects analysis of Wang tube sperm separating system in practice

作　　者：韦相才[1] 田佩玲[1] 陈平乐[1] 杨卫[1] 朱嘉铭[1] 

机构地区：[1]广东省计划生育科学技术研究所,510600

出　　处：《广东医学》2000年第5期375-376,共2页Guangdong Medical Journal

基　　金：广东省科委重点攻关项目! (编号 :962 2 0 50 - 0 3)

摘　　要：目的　探讨王氏管精子优选法在精子优选的应用 ,并与传统的精子上游法进行比较。方法　随机选择 5 7份精液 ,采用两种方法进行配对处理 ,比较两种方法分离前后、两者间精子活动率、形态以及精子顶体形态和精子染色质等参数的变化。结果　两种方法处理后的精子活动率、精子形态差异有显著性 (P <0 0 1) ,王氏管法处理后精子顶体完整率、正常染色质率亦显示出显著的差异 (P <0 0 1)。结论　王氏管精子优选法和精子上游法在精子处理中获得较好的精子质量 ,而王氏管精子优选法则获得更优质的精子 。Objective To study and compare swim-up and Wang tube techniques in sperm separation. Methods 57 samples of semen were treated by two methods. Several characteristics of semen including sperm count, motility, morphology, acrosin morphology and normal chromatin were analyzed. Results There was significant difference between pre-treatment and post-treatment in both methods in terms of sperm motility and morphology. There was also significant difference on acrosin morphology and normal chromatin chromatin in Wang tube method, but not in swim-up method. Conclusion Both Wang tube and swim-up methods improve sperm quality, but Wang tube is superior to swim-up, especially in recovery rate of arcosin morphology and normal-chromatin sperm. It is a promising method in sperm separation for use in insemination and in vitro fertilization to improve birth health quality.

关 键 词：精子优选 上游法 王氏管法 人工授精 

分 类 号：R321－33[医药卫生—人体解剖和组织胚胎学]