重组日本血吸虫副肌球蛋白的表达与纯化  

Expression and purification of recombinant Schistosoma japonicum paramyosin~①

作　　者：周金春[1] 易新元[1] Kalinna BH Mc Manus DP 

机构地区：[1]湖南医科大学血吸虫病研究,长沙410078 [2]The Queensland Institute of Medical Research

出　　处：《湖南医科大学学报》2000年第2期106-108,共3页Bulletin of Hunan Medical University

基　　金：WHO/TDR资助! ( 980 2 6 8)

摘　　要：用基因重组技术 ,将日本血吸虫副肌球蛋白 (rSj97)基因亚克隆至表达载体pQE30上。在IPTG诱导下 ,重组日本血吸虫副肌球蛋白在大肠杆菌中得以高效表达。通过快速蛋白质液相色谱仪 (FPLC) ,经TALON柱和离子交换柱两步分离纯化 ,获得大量高纯度的重组日本血吸虫副肌球蛋白 。Paramyosin of Schistosoma japonicum was expressed at a high level in E.coli. The recombinant protein could be easily purified from bacteria lysate by fast protein liquid chromatography(FPLC) on a TALON resin column, due to the protein being expressed with a tag of six histidine residue fused to the N terminus. The protein was completely soluble and could be eluted under non denaturing condition using imidazole. To eliminate imidazole and residue of E.coli, the elution was further purified by ion exchange chromatography. The purified protein will be used in water buffaloes in the study on protective immunity against Schistosoma japonicum. [

关 键 词：日本血吸虫病 副肌球蛋白 rSj97基因 亚克隆 

分 类 号：S852.4[农业科学—基础兽医学]