前列腺癌与良性前列腺增生细胞株差异核基质蛋白的鉴定  

Nuclear matrix proteins differentially expressed in human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line

作　　者：孙铁成[1] 辛玲[2] 宋黎明[3] 周越[2] 宁丽峰[2] 韩丽媛[1] 郭颖[1] 于和鸣[2] 王慧萍[2] 

机构地区：[1]北京协和医学院研究生院,北京100730 [2]国家人口计生委科学技术研究所,北京100081 [3]首都医科大学附属北京朝阳医院泌尿外科,北京100020

出　　处：《中华男科学杂志》2012年第7期583-589,共7页National Journal of Andrology

基　　金：中央级公益科研院所基本科研业务费专项(2009GJSSJKB07)~~

摘　　要：目的:鉴定人前列腺癌雄激素依赖性细胞株LNCaP、前列腺癌雄激素非依赖性细胞株PC-3与良性前列腺增生细胞株BPH-1之间差异表达的核基质蛋白(nuclear matrix proteins,NMPs)。方法:常规制备各细胞株NMPs,应用双向凝胶电泳对其进行分离;对差异表达的蛋白质点行MALDI-TOF-MS/MS质谱分析,数据库搜索并鉴定。结果:成功获得了分辨率高、重复性好的不同细胞株NMPs双向凝胶电泳图谱;初步鉴定出包含酶、调节蛋白、RNA结合蛋白及各种因子在内的12个差异表达的蛋白质,在癌细胞株中3个NMPs表达上调,9个下调。结论:人前列腺癌细胞与良性前列腺增生细胞之间NMPs表达存在明显差异;初步筛选出12个差异NMPs,其表达水平及功能与疾病的联系需进一步研究。Objective： To compare the expression of nuclear matrix proteins （NMPs） in benign prostatic hyperplasia （ BPH） epithelial cell line BPH-1 versus those in androgen-dependent human prostate cancer cell line LNCap and androgen-independent prostate cancer cell line PC-3. Methods ： We isolated NMPs from the BPH-1, LNCap and PC-3 cell lines by 2-dimensional electrophoresis （2-DE）, analyzed the differentially expressed proteins by matrix-assisted laser desorption / ionization time of flight mass sPectrometry （MALDI-TOF-MS）, and identified them by peptide mass fingerprint and database searching. Results： We successfully obtained well-resolved reproducible 2-DE patterns of NMPs in human prostate cancer cell lines, identified 12 differentially expressed NMPs ineluding enzymes, regulatory proteins, RNA-binding protein and various other factors, 3 up-regulated and 9 down-regulated in prostate cancer cell lines. Conclusion： There are obvious differences in the expressions of NMPs between human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line.

关 键 词：前列腺癌雄激素依赖性LNCaP细胞株 前列腺癌雄激素非依赖性PC-3细胞株 良性前列腺增生 BPH-1细胞株 核基质蛋白 

分 类 号：R737.25[医药卫生—肿瘤]