复方苦参凝胶节育与抗炎的体内实验研究  

In vivo study of antifertility and anti-inflammatory effects of compound Kucen gel

作　　者：缪建萍[1] 卢灿峰[1] 孟祥虎[1] 丁晶[2] 臧光辉[1] 苏倩[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030 [2]华中科技大学同济医学院药学院,湖北武汉430030

出　　处：《中华男科学杂志》2012年第7期656-660,共5页National Journal of Andrology

基　　金：中央高校基本科研业务费专项资金资助(2010MS125)~~

摘　　要：目的:研究复方苦参凝胶体内节育及抗炎的效果。方法:①节育实验:将60只雌性SD大鼠随机分为生理盐水组,空白凝胶组,复方苦参凝胶低(0.05 g生药/g)、中(0.10 g生药/g)、高剂量(0.15 g生药/g)组以及阳性对照(4%壬苯醇醚凝胶)组,每组10只。按上述分组雌鼠阴道内给予相应药物各200μl,然后雌雄大鼠按1∶1合笼,进行交配。在交配成功后的第12天剖腹观察雌鼠孕珠数,并计算避孕率;②抗炎实验:采用小鼠耳廓双面均匀涂抹二甲苯的致炎模型,将60只昆明种小鼠随机分为6组,分组同前,计算小鼠单侧耳的肿胀度及肿胀抑制率。结果:复方苦参凝胶高剂量组能显著抑制雌鼠的孕珠数,其孕珠数为0.00±0.00,显著低于生理盐水组(11.00±2.00,P<0.05),而与阳性对照组(0.00±0.00)比较无统计学意义(P>0.05),其避孕率为100%;在小鼠耳廓二甲苯致炎模型中,高剂量组同样能显著抑制其肿胀程度,其平均肿胀程度为10.17±2.56,显著低于生理盐水组(21.32±3.17,P<0.01),而与阳性对照组比较无统计学意义(8.53±1.89,P>0.05),其肿胀抑制率为52.3%。结论:复方苦参凝胶有较好的节育及抗炎作用,值得进一步开发并应用于临床。Objective： To study the antifertility and anti-inflammatory effects of compound Kucen gel in vivo. Methods： As antifertility experiment, we randomly divided 60 female SD rats into six groups of equal number： normal saline, blank gel, low- medi- um- and high-dose compound Kucen gel （0.05, 0.10 and 0.15 g/g）, and positive control （4% nonoxynol gel） to receive intravaginal administration of 200 μl of respective agent, followed by copulation with male rats in a 1：1 ratio. At 12 days after successful mating, the female rats were dissected for calculation of the embryos and the rate of contraception. As an anti-inflammatory trial, we established a mouse model of inflammation by applying xylene to the pinna, and equally randomized 60 Kunming mice to six groups as in the former experiment. We determined the degrees and average rates of swelling inhibition in the left ear. Results ： High-dose compound Kueen gel achieved a fertility-inhibition rate of 100% in the female rats, the number of embryos significantly lower than in the normal saline group （0.00 ± 0.00 vs 11.00± 2.00, P 〈 0.05 ）, but with no statistically insignificant difference from that of the positive control （0.00 ± 0.00, P 〉 0.05 ）. High-dose compound Kueen gel also markedly suppressed swelling in the left ear of the mice, with an inhi- bition rate of 52.3% , the average swelling degree significantly lower than in the normal saline group （ 10.17± 2.56 vs 21.32 ± 3.17, P〈0.01）, but not remarkably different from that of the positive control （8.53 ±1.89, P〉0.05）. Conclusion： Compound Kucen gel, with its strong antifertility and anti-inflammatory effects, deserves further study and clinical application.

关 键 词：复方苦参凝胶 节育 抗炎 避孕 二甲苯 壬苯醇醚 

分 类 号：R285.5[医药卫生—中药学]