多次低剂量链脲佐菌素诱导大鼠糖尿病模型的方法学研究  

Methodology research of rat diabetic model induced by streptozotocin several times

作　　者：李莉[1] 陈光亮[1] 韩茹[1] 朱克克[1] 汪俊[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,安徽合肥230038

出　　处：《安徽医药》2012年第7期888-890,共3页Anhui Medical and Pharmaceutical Journal

基　　金：国家中医药临床研究基地--糖尿病重点研究病种;安徽中医学院自然科学研究基金项目(No 2011ZR007B)

摘　　要：目的分别探讨造模后不同时间点禁食、大鼠性别和多次低剂量链脲佐菌素(STZ)对大鼠血糖及糖尿病模型成模率的影响。方法分别一次性腹腔注射(ip)STZ 30 mg.kg-160、62、64 h后,于晚8时、10时、12时禁食,次日晨8时测大鼠空腹血糖,计算糖尿病(空腹血糖≥7.0 mmol.L-1)成模率;记录雌鼠和雄鼠一次性ip STZ 30 mg.kg-1后糖尿病的成模情况;首次分别ip STZ(20、25、30、40和50 mg.kg-1),不成模大鼠再次ip STZ(20 mg.kg-1),比较各组间糖尿病成模情况、血糖水平和6周内大鼠死亡情况。结果晚10时和12时禁食组糖尿病成模数(均为8只)较晚8时禁食组成模数(4只)增加1倍,成模大鼠血糖值进一步升高;雄鼠糖尿病成模数(7只)较雌鼠成模数(4只)增加将近1倍,成模大鼠血糖值进一步升高;首次分别腹腔注射STZ 25、30、40和50 mg.kg-1各组大鼠累计成模率明显高于首剂20 mg.kg-1组;首剂50 mg.kg-1组6周内成模大鼠死亡率明显高于首剂STZ 25和30 mg.kg-1组。结论测大鼠空腹血糖前于晚10～12时禁食优于晚8时禁食;采用雄鼠造模优于雌鼠;首剂采用STZ 25～30 mg.kg-1腹腔注射,未成模大鼠继续采用STZ 20mg.kg-1腹腔注射,糖尿病成模率高,死亡率低。Objective To explore the effect of fasting time, sex difference and low dosage streptozotocin several times on diabetic model incidence in rats. Methods After ip STZ 30 mg .kg - l 60,62 and 64 h, rats were fasted at 20,22 and 24 o＇ clock separately, then to detect fasting plasma glucose （FPG） at 8 o＇ clock next moring , to caculate the diabetic model incidence （ FPG ≥ 7.0 mmol . L - 1 ） . Female and male rats were injected STZ 30 mg. kg-1 separately,to observe the diabetic model incidence difference. The male rats were ip STZ （20,25,30,40,50 mg . kg-1 ）separately, after 72h,those blood glucose not up to the standard were ip STZ 20 mg . kg-l again, to compare the incidence of diabetic model and the blood glucose level. Results The diabetic rats number of the groups fasted at 22 and 24 o＇ clock were double of that in the group fasted at 20 o＇ clock ; the blood glucose of diabetic rats rose further. The male diabetic rats number was nearly double of that in female group, the blood glucose of diabetic rats rose further too. The accumulated diabetic model incidences of rats injected STZ 25,30,40 and 50 mg . kg-1 separately at the first time were higher than the group injected STZ 20 mg .kg i at the first time（50% ）, the death incidence of the group injected STZ 50 mg· kg-1 at the first time was higher than the groups injected STZ 25,30 mg · kg-1 at the first time in 6 weeks. Conclusion Before detecting FPG the best fasting time was 22 - 24 o＇ clock. The absorption of male rats to make diabetic model was better than female rats . The best method to establish diabetic model of rats with high incidence and low ineiednee of death is ip STZ 25 · 30 mg · kg- 1 at the first time , then the rats whose blood glucose was not up to the standand received ip STZ 20 mg · kg-1 again.

关 键 词：糖尿病模型 链脲佐菌素 大鼠 

分 类 号：R965[医药卫生—药理学]