鼻咽癌组织中EB病毒的不同原位检测方法比较  

Comparison of different methods for in situ detection of Epstein-Barr virus in nasopharyngeal carcinoma

作　　者：许亮国[1] 姚开泰[1] 谢鹭[1] 周文 杨玉文 陈曲侯 王珣章[2] 

机构地区：[1]湖南医科大学卫生部癌变原理重点实验室,湖南长沙410078 [2]中山大学生命科学院,广东广州510275

出　　处：《癌症》2000年第1期31-33,共3页Chinese Journal of Cancer

基　　金：国家自然科学基金重点项目资助!(No.39730200)

摘　　要：目的：探讨在鼻咽癌组织石蜡切片标本中原位检测ＥＢ病毒的更快速、更灵敏的方法。方法：用地高辛标记的寡核苷酸ＥＢＥＲ－１和ＥＢＶ ＤＮＡ的 ＢａｍＨＩ－Ｗ片段为探针，分别用原位杂交、原位ＰＣＲ检测鼻咽癌组织切片中ＥＢＶ的分布情况。结果：用寡核苷酸ＥＢＥＲ－１作探针原位杂交检测ＥＢ病毒的方法比用ＢａｍＨＩ－Ｗ片段作探针原位杂交及原位ＰＣＲ的方法更灵敏、更简单。结论：用ＥＢＥＲ－１作探针原位杂交检测鼻咽癌组织切片中ＥＢ病毒的方法不失为一种简单、快速、灵敏的方法。Objective: To investigate a rapid, sensitive non-isotopic method for in situ detection of Epstein-Barr virus (EBV) in paraffin-embedded section of nasopharyngeal carcinoma (NPC) tissues. Methods: The oligo-nucleotide EBER-1 and EBV BamHI-W fragment were labeled with digoxigenin as probes, in situ hybridization and PCR in situ hybridization were performed to detect EBV distribution in NPC tissues. Results: The signal strength of in situ hybridization with EBER-1 oligonucleotide probe was clearly stronger than that of in situ hybridization and PCR in situ hybridization with BamHI-W fragment probe. Conclusion: In situ hybridization with EBER-1 probe to detect EBV in NPC tissues is a sensitive, simple and rapid method.

关 键 词：原位杂交 EBER-1 EB病毒 鼻咽肿瘤 

分 类 号：R739.6[医药卫生—肿瘤]