突触数密度Disector测量法的建立与计算机辅助分析的实现  

THE SYNAPTIC NUMERICAL DENSITY COUNTING BASED ON DISECTOR TECHNIQUE AND ITS COMPUTER-AIDED ANALYSIS

作　　者：张琳[1] 买鸿宴[1] 黄浩[2] 刘贺飞 南燕[1] 尚俊杰 廖洪恩 于恩华[1] 沈丽[1] 

机构地区：[1]北京医科大学解剖学系,北京100083 [2]北京大学力与工程科学系,北京100871

出　　处：《解剖学报》2000年第1期8-12,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金!( 3 9470 3 0 9);卫生部科研基金!( 98-1-2 63 )

摘　　要：目的　建立基于 Disector技术的突触数密度定量研究方法 ;利用 Visual Basic语言实现计算机辅助的突触数密度定量分析。　方法　应用连续超薄切片技术和透射电镜技术观察并记录位于同一纵轴上的神经毡 ,选取已知距离的相邻平行电镜照片构成 Physical Disector,依据 Disector原理计算突触的数密度 ;以 Visual Ba-sic4.0为开发工具 ,在 Windows95平台上开发基于 Disector技术的突触数密度计算机辅助分析系统 (DisectorCountor 1.0 )。　结果　比较手工计数与计算机辅助分析的结果表明两者无显著性差异 (P>0 .0 5 )。　结论　我们开发的基于 Disector技术的突触的数密度计算机辅助分析系统—— Disector Countor 1.0 ,具备客观、高效等优点 ,是突触数目定量分析的发展方向。Objective\ We developed a quantitative method based on the Disector technique to count the synaptic numerical density and implemented the computer\|aided analysis on it with the Visual Basic.Methods\ The neuropils on the same vertical axes were observed and recorded with the serial ultrathin section technique as well as transmission electron microscopy.The adjacent parallel electron micrographs of known distance apart were selected to make up the Physical Disector,and the synaptic numerical density was counted according to the Disector principle.The computer\|aided analysis system for synaptic numerical density based on the Disector technique(DisectorCountor 1\^0) was developed on the Windows95 system with the Visual Basic 4\^0.Results\ There was no significant statistical difference ( P >0 05) between the result by hand and that with Disector Countor 1\^0.Conclusion\ With the advantage of being highly objective and efficient,the computer\|aided analysis system for synaptic numerical density based on the Disector technique,Disector Countor 1\^0,would be the developmental direction for synaptic numerical density analysis.

关 键 词：突触 数密度 体视学 DISECTOR 计算机辅助分析 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学] R338.13[医药卫生—基础医学]