皖南花猪不同发育阶段脂肪组织脂联素及受体和瘦素mRNA的变化  

Changes in the mRNA expression of adiponectin,adiponectin receptors,and leptin in adipose tissue of Wannanhua pigs at different stages of development

作　　者：盛晟[1] 周杰[1] 张佳[1] 邵康[1] 吴小雪[1] 李维新[1] 殷宗俊[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《中国应用生理学杂志》2012年第4期352-355,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金项目(30771581);安徽省十一五科技攻关计划重大科技专项(08010301079)

摘　　要：目的:研究皖南花猪不同发育阶段不同部位脂肪组织中脂联素(Adp)及其受体(AdpR1、AdpR2)和瘦素(leptin)mRNA的变化及性别差异。方法:选择出生、30、45、90、180日龄的皖南花猪雌、雄各5头,以β-actin为内标,采用△△Ct相对定量实时荧光PCR方法对皮下脂肪和肾周脂肪中Adp、AdpR1、AdpR2和leptin mRNA进行定量分析。结果:不同发育阶段皮下脂肪和肾周脂肪Adp、AdpR1、AdpR2、leptin mRNA的表达都有极显著差异(P<0.01)。总体上Adp mRNA在肾周脂肪显著高于皮下脂肪(P<0.05);AdpR1、AdpR2和leptin mRNA在皮下脂肪显著或极显著高于肾周脂肪(P<0.05或P<0.01)。除个别基因和个别日龄外,总体上各基因mRNA表达的性别差异不明显。无论在皮下脂肪还是肾周脂肪,Adp mRNA的表达与AdpR1、AdpR2呈显著或极显著正相关(P<0.05或P<0.01),与leptin显著负相关(P<0.05)。结论:皖南花猪不同发育阶段脂肪组织中Adp、AdpR1、AdpR2、leptin的基因表达有差异,且有组织特异性;Adp与其受体mRNA表达有相关性。Objective： To explore the changes in the mRNA expression of adiponectinc（Adp）, adiponectin receptors（AdpR）, and leptin in different adipose tissues of Wannanhua pigs at different stages of development, and their sexual dimorphism. Methods： Five Wannanhua boars and five Wannanhua gilts were sampled at birth, 30, 45, 90, and 180 days of age respectively. The △△Ct relative quantification realtime PCR was used to detect the transcription levels of Adp, AdpR1, AdpR2, and leptin mRNAs in subcutaneous（SC） and perirenal（PR） adipose tissues, and β-actin were used as internal standards. Results： The expression level of Adp, AdpR1, AdpR2, and leptin mRNA in SC and PR adipose tissue were changed with age significantly （ P 〈 0.01）. In general, Adp mRNA expression in SC adipose tissue was significandy lower than that in PR adipose tissue （ P 〈 0.05）, while AdpR1, AdpR2, and leptin mRNA expression in SC adipose tissue were significantly higher than those in PR adipose tissue （P 〈 0.05 or P 〈 0.01 ）. Although the sexual dimorphism were found in apart genes or apart days of age, Adp, AdpR1, AdpR2, and leptin mRNA expression both in SC adipose tissue and PR adipose tissue had no significant differences between Wannanhua gilts and boars in general. Significant positive correlation was found between Adp and AdpR1, AdpR2 （ P 〈 0.05 or P 〈 0.01）, and significant negative correlation was found between Adp and leptin （ P 〈0.05） in SC adipose tissue and PR adipose tissue respectively （ P 〈0.05）. Conclusion： The expression of Adp, AdpR1, AdpR2, and leptin mRNA in adipose tissue of Wannanhua pigs followed specific developmental patterns and tissue specificity. Adp correlated with its receptors.

关 键 词：皖南花猪 脂联素 脂联素受体 脂肪组织 发育性变化 

分 类 号：S828.8[农业科学—畜牧学] Q955[农业科学—畜牧兽医]