内异方药物血清对子宫内膜异位症血管生成的影响  

Effects of Chinese herbal medicine Neiyi Recipe-medicated serum on angiopoiesis of endometriosis in the chick chorioallantoic membrane model

作　　者：徐玲[1] 周巧玲[1] 韩洁[1] 张丹英[1] 董惠娟[1] 蔡在龙[2] 俞超芹[1] 翟东霞[1] 

机构地区：[1]第二军医大学长海医院中医科,上海200433 [2]第二军医大学长海医院实验中心,上海200433

出　　处：《中西医结合学报》2012年第7期800-806,共7页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金重点项目(No.30930113);国家自然科学基金资助项目(No.30371838;30672742;30873353)

摘　　要：目的:比较子宫内膜异位症(endometriosis,EM)在位、异位内膜组织与对照在位内膜组织的血管生成能力及血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达水平,并评价中药内异方药物血清对其的影响。方法:采用鸡胚尿囊膜(chick chorioallantoic membrane,CAM)上子宫内膜组织种植的方法建立EM模型。Sprague-Dawley大鼠分别灌胃生理盐水、达那唑和内异方制备对照血清和药物血清。将CAM模型分别用空白血清、达那唑药物血清和内异方药物血清干预后,测量种植灶面积和阳性血管面积,计算阳性血管面积百分比;免疫组织化学EnVision法检测各组种植组织中VEGF的表达。结果:EM患者的在位、异位内膜组织种植于CAM后,种植灶面积与对照在位内膜组比较差异无统计学意义(P>0.05),但EM异位内膜种植灶周围形成的血管面积显著高于在位内膜种植后(P<0.05);与各自空白血清组相比,内异方血清组的EM在位、异位内膜组织种植于CAM后形成的种植灶面积明显缩小(P<0.01,P<0.05),种植灶周围阳性血管面积显著减少(P<0.01)。达那唑与内异方作用相似,但其异位内膜种植灶面积与空白血清组比较,差异无统计学意义(P>0.05)。EM在位、异位内膜组种植灶中VEGF的阳性表达显著高于对照在位内膜组(P<0.01),内异方血清组VEGF的阳性表达显著低于各组空白血清组(P<0.01),达那唑血清组亦下降明显,但异位组织与空白血清组比较差异无统计学意义(P>0.05)。结论:EM在位、异位内膜组织中VEGF的表达水平明显增高,异位内膜组织血管形成能力显著增强。内异方药物血清可显著降低EM在位和异位内膜组织中VEGF的表达,抑制种植灶新生血管网的形成,减少异位灶局部血供,从而抑制异位内膜组织的生长。与达那唑作用相比,中药内异方在抑制和治疗EM上有相似的作用,但在抑制异位内膜组织VEGF的表达方面,中药内异方作用更�OBJECTIVE：To compare angiopoiesis ability of eutopic and ectopic endometrial tissue isolated from women with endometriosis and endometrium isolated from women without endometriosis（control）,and to explore the inhibitory effects of medicated serum of Neiyi Recipe,a compound traditional Chinese herbal medicine. METHODS：Chick chorioallantoic membrane（CAM）model of endometriosis was established by transplanting endometrium onto CAM.The CAMs were then hatched with blank serum or medicated serum of danazol or Neiyi Recipe,which were prepared in rats by orally administering.The sizes of the transplanted tissue and new vessels around the transplanted tissue were measured.Expression of vascular endothelial growth factor （VEGF）was detected by immunohistochemical method. RESULTS：There was no difference in the sizes of transplanted tissue among CAM models of ectopic and eutopic endometrial tissue isolated from women with endometriosis or control（P0.05）,and more new vessels were found around the ectopic and eutopic endometrial tissue than the endometrial tissue of control （P0.05）.Compared with the controls,the size of the transplanted tissue and positive area of new vessels were significantly inhibited by Neiyi Recipe-medicated serum（P0.01,P0.05）,and similar changes happened in the danazol groups,except for the size of transplanted tissue from ectopic endometrial tissue （P0.05）.Expression of VEGF was significantly higher in eutopic and ectopic endometrial tissue than in the control（P0.01）;the level of VEGF obviously reduced in the Neiyi Recipe and danazol groups（P0.01）, but no significant difference was detected between them. CONCLUSION：Endometrium from women with endometriosis stimulates the formation of new vessels by increase the expression of VEGF.Neiyi Recipe-medicated serum significantly decreases the expression of VEGF in eutopic and ectopic endometrial tissues and thus restrains the formation of new vessels,reduces the blood supply and inhibits growth of ectopic endometrial t

关 键 词：子宫内膜异位症 鸡胚尿囊膜 血管内皮生长因子 中草药 血管生成抑制剂 

分 类 号：R711.71[医药卫生—妇产科学]