羊膜腔内注射肺表面活性物质对宫内感染致肺损伤胎兔肺表面活性相关蛋白A表达的影响  

Effect of intra-amniotic administration of pulmonary surfactant on expression of SP-A in fetus lung injury in rabbit model with intrauterine infection

作　　者：吴静[1,2] 刘冬云[3] 刘敬[2] 封志纯[2] 

机构地区：[1]南方医科大学附属何贤纪念医院新生儿科,广州511400 [2]北京军区总医院附属八一儿童医院新生儿中心,100700 [3]青岛大学医学院附属医院新生儿科,山东青岛266003

出　　处：《检验医学与临床》2012年第13期1565-1567,1569,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨羊膜腔内注射肺表面活性物质(PS)对宫内感染致肺损伤胎兔肺组织肺表面活性相关蛋白A(SP-A)表达的影响。方法选取健康成年育龄日本大耳白兔19只,成功受孕后随机分为对照组、细菌组、细菌+PS组。建立宫内感染模型。对照组和细菌组按胎龄又分为21、22、23、24、26d5个亚组,细菌+PS组分为24d、26d2个亚组。细菌组宫内注射大肠埃希杆菌(E.coli)菌株,细菌+PS组宫内注射E.coli菌株,同时胎兔羊膜腔内注射PS 200mg/kg,对照组宫内注射生理盐水。模型建成后分别在各时间点对孕兔行剖宫产,杀取胎兔后取肺组织,以TGF-β1兔多克隆抗体为一抗,用免疫组织化学方法检测其肺组织内SP-A表达;用RT-PCR检测其SP-A mRNA表达;用Western blotting方法检测其SP-A蛋白表达;应用SPSS13.0软件进行统计学处理。结果免疫组化结果显示不同组间SP-A表达差异有统计学意义(F=237.865,P=0.000),细菌组SP-A表达明显低于对照组及细菌+PS组。SP-A mRNA、蛋白表达则在细菌感染后明显下降,差异具有统计学意义(F=1 101.741,P=0.000)。注射PS后SP-A表达增强,高于细菌组(P=0.000),与对照组比较差异无统计学意义(P>0.05)。结论羊膜腔内感染可导致SP-A异常低表达,与新生儿呼吸窘迫综合征(RDS)发生密切相关;羊膜腔内注射PS后SP-A表达上调,表明羊膜腔内注射PS可做为一种安全、有效的预防治疗手段,对降低新生儿发生呼吸窘迫综合征等肺疾病具有重要意义。Objective To investigate effect of intra-amniotic administration of pulmonary surfactant on expression of SP-A in fetus lung injury in rabbit model with intrauterine infection.Methods Nineteen Japanese albino pregnant rabbits were divided into 3 groups： bacteria group,bacteria ＋ PS group and control group.The rabbit models of fetus lung injury with intrauterine infection were established.In the bacteria group,the rabbits were injected with intrauterine intestinum crassum bacterium,and then were subdivided into 21 d,22 d,23 d,24 d,26 d fetation group respectively;in the bacteria ＋ PS group,the rabbits were injected with intrauterine bacterium coli commune and amniotic intracavitary administration extrinsic source PS 200 mg/kg,and then were subdivided into 24 d and 26 d fetation group respectively.In the control group,the rabbits were injected with 0.9% sodium chloride,and then were subdivided with the same criteria as that in the bacteria group.All the rabbits were delivered by cesarean section and the fetus were taken.Lung tissues of fetus rabbits from each rabbit were excised respectively.Immunohistochemistry method was used to detect SP-A expressions in lung tissues.The expression of SP-A mRNA in lung tissues were detected by Reverse transcription-Polymerase chain reaction and SP-A protein by Western blot.Data were analyzed by SPSS 13.0 software.Results Compared with the control group,the expression of SP-A in bacteria group was significantly decreased,with significant differences（F=237.865,P=0.000）.The expression of SP-A mRNA and protein in bacteria group was significantly lower than those of the control group（F=1 101.741,P=0.000）,which in PS group was higher than bacteria groups（P〉0.05）.Conclusion The expression of SP-A decreased after infection,and intracavitary administration PS can lead to the increase of SP-A,which indicate that replacement therapy of PS can increase the expression of SP-A,and this is a useful method to prevention NRDS for postnatal.

关 键 词：肺表面活性物质 宫内感染 肺损伤 肺表面活性相关蛋白A 

分 类 号：R722.12[医药卫生—儿科]