生化检验分析中肝素钠抗凝血浆应用的可行性分析  

The feasibility analysis of sodium heparin anticoagulative plasma′s application in biochemical test

作　　者：唐继海[1] 黄文方[1] 李峥嵘[1] 刘蔚[1] 程余恒[1] 

机构地区：[1]四川省医学科学院/四川省人民医院检验科,成都610071

出　　处：《检验医学与临床》2012年第13期1589-1590,共2页Laboratory Medicine and Clinic

摘　　要：目的对生化检验分析中肝素钠抗凝血浆应用的可行性进行研究分析。方法对肝素钠抗凝血浆以及血清的10项生化项目的测定结果作相关比较,并提出对应的处理方法,选择20例本院门诊的患者,按照常规的方法抽取静脉血液4mL,各取一半分别注入2支不同的试管。其中一支试管不做抗凝处理,放置于37℃并水浴30min,然后通过离心分离出血清;另一试管含肝素钠抗凝剂,在加血之后立刻进行离心分离出血浆。钾(K+)、钙(Ca2+)、钠(Na+)、氯(Cl-)、镁(Mg2+)、葡萄糖(Glu)、血尿素氮(BUN)、肌酐(Cr)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST),以上10个项目指标均使用日立7080型的全自动分析仪来进行测定,并对结果进行记录。结果血清和血浆中Cl-、Ca2+、Mg2+、BUN、Cr、ALT、AST的检验结果基本一致;钠和葡萄糖差异有统计学意义(P<0.05);与钾差异也有统计学意义(P<0.01),血清钾浓度(Y)与血浆钾浓度(X)的回归方程为Y=0.715 X+0.499,r=0.749。结果清晰地表明了血清跟血浆中的K+、Glu、Na+测定值差异具有统计学意义(P<0.05),而其它的项目指标之间的差异无统计学意义(P>0.05)。结论采用肝素钠抗凝血浆进行生化检验分析,只要纠正了钾的差异或者可以考虑建立一个血浆钾参考值的范围即可迅速,准确地报告出结果,为协助临床医生的及时合理用药提供了技术保障。Objective To analyze the feasibility of sodium heparin anticoagulative plasma′s application in biochemical test.Methods We compared the determination results of heparin anticoagulant plasma and 10 biochemical items of serum,and put forward the corresponding treatment methods.20 outpatients from our hospital were collected.We extracted 4 mL of venous blood according to the conventional method,which were separately into 2 test tubes.One tube did not do the anticoagulant treatment,placed in 37 ℃ water bath for 30 minutes,then we centrifuged serum.Another test tube did the sodium heparin anticoagulant treatment,then we centrifuged serum immediately.The levels of K＋,Ca2＋,Na＋,Cl＋,Mg2＋,Glu,Bun,Cr,ALT,AST were tested by using the Backman type LX20 automatic analyzer,and the results were recorded.Results serum and plasma levels of calcium,magnesium,chloride,urea nitrogen,creatinine,alanine transaminase,aspartate amino transferase examination results were basically the same;the result of sodium and glucose′s level was slightly different（P〈0.05）;and the result of potassium′s level was obvious different（P〈0.01）,the serum potassium concentration（Y） and plasma potassium concentration（X） of the regression equation was Y=0.715X＋0.499,r=0.749.The results clearly showed that the serum and plasma in K＋,Glu,Na＋ determination value were significantly different（P〈0.05）,there was no significant difference in other project indicators（P〉0.05）.Conclusion The sodium heparin anticoagulative plasma′s application in biochemical test analysis,as long as we correct potassium differences or build a plasma potassium reference value range,can be quickly,accurately report the results,which provide technical support to assist clinicians in rational drug use.

关 键 词：生化检验 分析 肝素钠 抗凝血浆 应用 

分 类 号：R446.112[医药卫生—诊断学]