COX-2抑制剂在大鼠胰十二指肠移植缺血再灌注损伤中对P选择素和细胞间粘附分子-1的影响  

Effect of cyclooxygenase-2 inhibitor on P-selectin and intercellular adhesion molecule 1 in rats with ischemia/reperfusion injury following pancreaticoduodenal transplantation

作　　者：赵雪云[1] 张力[1] 刘胜春[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌外科,重庆400016

出　　处：《第三军医大学学报》2012年第14期1380-1383,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30871295)~~

摘　　要：目的探讨特异性环氧合酶-2抑制剂(cyclooxygenase-2 inhibitor,COX-2 inhibitor)NS398能否抑制大鼠胰十二指肠移植缺血再灌注损伤中P选择素(P-selectin,Ps)及细胞间粘附分子-1(intercellular adhesion molecule-1,ICAM-1)的表达。方法采用单纯随机抽样的方法将75只SD大鼠分为3组:假手术组(15只)、移植组(15对)、移植+NS398组(15对)。各组均于术后1、3、6 h取血测定血清淀粉酶含量,光镜下观察胰腺病理改变,免疫组织化学法和RT-PCR法分别检测胰腺组织中ICAM-1及Ps的表达情况和基因水平的变化。结果假手术组各时间点胰腺组织损伤不明显,血淀粉酶不升高(P>0.05);随再灌注时间的延长,移植组胰腺组织损伤加重,血淀粉酶较同时点假手术组明显升高(P<0.05);但与移植组比较,移植+NS398组胰腺组织损伤及血淀粉酶升高均较轻(P<0.05)。假手术组各时点Ps和ICAM-1均不表达;移植组、移植+NS398组各时点Ps和ICAM-1均有表达,但移植+NS398组Ps和ICAM-1的表达均明显低于移植组(P<0.05)。结论 NS398能抑制胰十二指肠移植缺血再灌注损伤大鼠的Ps及ICAM-1表达,对大鼠胰十二指肠移植缺血再灌注损伤有保护作用。Objective To study whether specific cyclooxygenase-2 （COX-2） inhibitor NS398 can in- hibit the expression of P-seleetin （Ps） and intercellular adhesion molecule 1 （ICAM-1） in rats with ischemia/ reperfusion （I/R） injury following pancreaticoduodenal transplantation. Methods Seventy-five SD rats were randomly divided into sham operation group （n = 15 ）, transplantation group （n = 30）, and transplantation ＋ NS398 group （n = 30）. Blood samples were taken from 3 groups at 1, 3 and 6 h after operation. Serum amy- lase level was measured. Pancreatic lesion was observed under light microscope. Expression of Ps and ICAM-1 in pancreatic tissue was detected by RT-PCR and immunohistochemistry, respectively. Results The pancrea- tic tissue injury was not significant and the serum amylase level was not elevated in sham operation group at dif- ferent time points （ P 〉 0. 05 ）, which were aggravated with the prolongation of reperfusion （ P 〈 0. 05 ）. The pancreatic tissue injury was severer and the serum amylase level was lower in transplantation ＋ NS398 group than in transplantation group （ P 〈 0. 05 ）. Ps and ICAM-1 were not expressed in sham operation group but ex- pressed in transplantation group and transplantation ＋ NS398 group at different time points. However, the ex- pression levels of Ps and ICAM-1 were significantly lower in transplantation ＋ NS398 group than in sham opera- tion group （P 〈 0.05）. Conclusion NS398 can inhibit the expression of ICAM-1 and PS in rats with I/R in- jury and protect rats against I/R injury following pancreaticoduodenal transplantation.

关 键 词：胰腺移植 缺血再灌注损伤 环氧合酶-2抑制剂 P选择素 细胞间粘附分子-1 

分 类 号：R656.64[医药卫生—外科学]