经脐静脉超声造影对晚孕胎鼠脑组织超微结构影响的初步研究  

The primary study on effects of ultrastructure of the brain of the late trimester fetal rats via transumbilical veincontrast-enhanced ultrasonography

作　　者：胡宪八[1] 盛常睿[1] 何慧疗[1] 张毅[1] 王亮[1] 黄品同[1] 陈成春[2] 

机构地区：[1]温州医学院附属第二医院超声科,325027 [2]温州医学院解剖实验室

出　　处：《中华超声影像学杂志》2012年第7期617-620,共4页Chinese Journal of Ultrasonography

基　　金：基金项目：浙江省自然科学基金面上项目（y2090376）;温州市科技局项目（y20110111）

摘　　要：目的观察经脐静脉超声造影对晚孕SD胎鼠脑组织超微结构的影响。方法40只晚孕SD大鼠随机分为4组，每只孕鼠子宫中随机选取2只经脐静脉穿刺成功后的胎鼠，注射生理盐水或造影剂声诺维（SonoVue）（每组胎鼠数20只）。A组：对照组，注射生理盐水120μl／kg；B组：低剂量组，注射SonoVue剂量为0．014mg／kg（120μl／kg，0．118mg／m1）；C组：中剂量组，注射SonoVue剂量为0．14mg／kg（120μ1／kg，1．18mg／m1）；D组：高剂量组，注射SonoVue剂量为1．4mg／kg（120μl／kg，11．8mg／m1）。观察各组各胎鼠的造影过程，造影后取各胎鼠的脑组织进行电镜检查。结果①A组各胎鼠及胎盘组织未见显影；B组在注射造影剂后迅速消退，视觉效果不理想；C组及D组可见胎鼠及胎盘依次先后显影。孕鼠子宫及子宫外组织始终未见显影。②电镜下：A组及B组脑细胞超微结构未见明显改变；C组及D组细胞胞质内均出现不同程度的粗面内质网扩张、线粒体肿胀、血管内皮细胞基底膜增厚、糖原沉积及空泡形成等超微结构改变，但C组超微结构较D组改变轻，与D组比较差异有统计学意义（P〈0．05）。结论经脐静脉超声造影对晚孕胎鼠脑组织超微结构有一定的影响，影响随造影剂剂量的减低而变小，但低剂量组视觉效果不理想。Objective To observe the ultrastructure changes of brain of the late trimester of fetal rats via transumbilical vein injection using contrast-enhanced ultrasonography. Methods The forty pregnant rats in the late trimester were divided into four experimental groups randomly. After being successfully punctured in the umbilical vein, two fetus was randomly chosen from maternal uterus with injection contrast agent SonoVue or normal saline solution（20 fetus/each group）. Group A as control group with injection of normal saline solution （120μl/kg） ;group B as low dose group with injection of SonoVue 0. 014 mg/kg （120 μl/kg,0. 118 mg/ml） ;group C as media close group with injection of SonoVue 0.14 mg/kg （120 μl/kg,1.18 mg/ml） and group D as high dose group with injection of SonoVue 1.4 mg/kg （120μl/kg, 11.8 mg/ml）. After observing the process of every contrast-enhanced ultrasonographic examination, tissue slices of brain of these fetus were made for electronmicroscope investigation. Results OThere was no enhancement in the fetus and placenta in group A. Enhancement couldn＇t be clearly seen in group B because of fast wash-out contrast agent. On the contrary, the fetus and placenta enhancement in turn could be seen in group C and group D. Enhancement never be seen in maternal uterus and uterine outside. There was no significant difference of ultrastructure changes of the fetal brain tissues in group A and group B with electronmicroscope. Different degree rough endoplasmic reticulum enlargement, mitochondria swelling, endothelial cell injury, glycogen deposition and vacuolization in the brain cells were found in group C and group D, and the value of contrast between the two groups was significantly different （P 〈0.05）. Conclusions It is decreasd gradually effects to perform transumbilical vein contrast enhanceduhrasonography with reducing gradually dose for the late trimester of fetal rats, but enhancement has been invisible in the low dose group.

关 键 词：超声检查 微气泡 胎盘 脑 

分 类 号：R445.1[医药卫生—影像医学与核医学]