腹泻性贝毒及其高效液相色谱检测方法  

Analysis of Diarrhetic Shellfish Poisoning Toxins by High Performance Liquid Chromatography

作　　者：陈则玲[1] 付云娜[1] 巩宁[2] 

机构地区：[1]国家海洋环境监测中心,大连116023 [2]大连水产学院农业部海水增养殖生态学重点开放实验室,大连116023

出　　处：《海洋通报》2000年第1期73-78,共6页Marine Science Bulletin

摘　　要：本文对赤潮中某些藻类所产生的腹泻性贝毒（ＤＳＰ）的来源、特性、化学结构以及对人体的危害及其测定方法进行了介绍。重点叙述了目前国际上迅速发展的高效液相色谱法测定ＤＳＰ的优点与其它测定方法的对比，并对我国ＤＳＰ发生的现状和检测标准作了概述。Diarrhetic Shellfish Poisoning (DSP)is one of the major toxins. It has been recognized in Japan and Europe since its discovery in the late 1970s. The main toxins responsible for DSP are Okadaic acid (OA)、DTX-1、DTX-2 and DTX-3, the last being a complex mixture of 7-O- acylderivatives of the first three. The most common method for detecting DSP toxins is the mouse bioassay. Recently, a liquid chromatography (LC)-linked phosphatase radioassay LC has been developed. Combined with ion-spray mass spectrometry (LC-MS) is a powerful method that is particularly well suited to the confirmation of toxin identities and the identification of new toxins. Especially, High performance liquid chromatography has made an important breakthrough in the extraction and determination of DSP.

关 键 词：腹泻性贝毒 毒素 高效液相色谱 赤潮 灌类 

分 类 号：R996.3[医药卫生—毒理学] X55[医药卫生—药学]