百日咳攻击菌液氮保存毒力稳定性的动态监测  

Dynamic monitoring of virulence stability of challenge strain of B. pertussis stored in liquid nitrogen

作　　者：王丽婵[1] 卫辰[1] 张路民[1] 徐颖华[1] 骆鹏[1] 张庶民[1] 侯启明[1] 

机构地区：[1]中国食品药品检定研究院血清室卫生部生物技术产品检定方法及其标准化重点实验室,北京100050

出　　处：《中国生物制品学杂志》2012年第7期909-910,共2页Chinese Journal of Biologicals

摘　　要：目的动态监测百日咳疫苗效价检定用攻击菌在液氮中保存的毒力稳定性。方法百日咳菌种复苏、培养3代后收集菌苔,比浊稀释后冻存。2008～2011年,每年采用改良脑腔攻击试验进行菌毒力的动态监测,分析其毒力稳定性。结果2008～2011年,制备的百日咳攻击菌于液氮中保存,4年中毒力无明显变化(P>0.05),稳定性良好。结论百日咳攻击菌液氮保存毒力稳定,可用于百日咳疫苗效力试验攻击菌的保存。Objective To dynamically monitor the stability of virulence of challenge strain, stored in liquid nitrogen, for de- termination of potency of pertussis vaccine. Methods After B. pertussis seeds was resuscitated and subcuhured for 3 passages, the bacterial lawns were collected, diluted by turbidimetry, distributed and stored in liquid nitrogen. The virulence of the B. pertussis strain was monitored dynamically every year from 2008 ~ 2010 by modified challenge test in mouse brain, of which the stability was evaluated. Results No significant change was observed in the virulence of challenge strain of B. pertussis after storage in liquid ni- trogen, idnicating high stability. Conclusion The virulence of challenge strain of B. pertussis was stable after storage in liquid nitro- gen, indicating that liquid nitrogen was suitable for storage of challenge strain of B. pertussis for potency test of pertusssis vaccine.

关 键 词：百日咳 攻击菌 液氮 稳定性 

分 类 号：R378.42[医药卫生—病原生物学]