MMP-2、TIMP-1和TIMP-2在胚胎停育绒毛和蜕膜组织中的表达  

Expression of Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-1,2 Chorionic Villi and Deciduas Tissues from Early Embryo Growth Arrest

作　　者：马媛[1] 王珺[1] 李博[1] 雷洁[1] 李怡[1] 巨瑛[1] 丁迎春[1] 王晓红[1] 

机构地区：[1]第四军医大学唐都医院妇产科,西安710038

出　　处：《生殖与避孕》2012年第7期452-456,469,共6页Reproduction and Contraception

摘　　要：目的:探讨MMP-2,TIMP-1和TIMP-2与胚胎停育的关系。方法:选取胚胎停止发育妇女为病例组,另设同期正常早孕自愿要求流产的妇女为对照组,分别留取其绒毛和蜕膜组织标本,采用半定量RT-PCR法对标本MMP-2,TIMP-1和TIMP-2 mRNA转录水平进行分析。结果:在绒毛标本中,MMP-2和TIMP-1 mRNA在病例组中表达水平显著低于对照组(P<0.01);而TIMP-2mRNA表达水平组间差异无统计学意义(P>0.05)。在蜕膜标本中,病例组TIMP-1 mRNA的表达水平显著低于对照组(P<0.01)。而MMP-2和TIMP-2 mRNA的表达水平组间差异无统计学意义(P>0.05)。结论:MMP-2,TIMP-1和TIMP-2的差异表达可能参与了胚胎停育的发生发展。Objective： To examine the expression of matrix metalloproteinase-2 （MMP-2）, tissue inhibitor of metalloproteinase-l（TIMP-1）, and TIMP-2 mRNA in chorionic villi and deciduas tissues from early embryo growth arrest. Methods： The trophoblastic and deciduas tissues were collected from women who were normal early pregnant （control group, n=58） and with early embryo growth arrest （case group, n=54）. Reverse transcriptase-ploymerase chain reaction （RT-PCR） was employed to detect expression levels of MMP-2, TIMP-1 and TIMP-2 mRNA. Results： In chorionic villi tissues, there was no significant difference in TIMP-2 mRNA expression level between the two groups, whereas the expression levels of MMP-2 and TIMP-1 mRNA in case group were significantly lower than those in the control （P〈0.01）. Meanwhile in deciduas tissues, there was no significant difference in MMP-2 and TIMP-2 mRNA expression level between the two groups. Whereas the mRNA expression level of TIMP-1 in case group was significantly lower than that in the control （P〈0.01）. Conclusion： The down-regulated expression of MMP-2 and TIMP- 1 in chorionic villi or/and deciduas tissues may contribute to the early embryo arrest.

关 键 词：胚胎停育 基质金属蛋白酶(MMPs) 基质金属蛋白酶组织抑制剂(TIMPs) 

分 类 号：R714.21[医药卫生—妇产科学]