‘短柄樱桃’花芽休眠解除过程中差异表达基因的研究  

Studies on Differentially Expressed Genes in Flower Bud During Dormancy Release in Prunus pseudocerasus Lindl.‘Duanbing’

作　　者：李永强[1] 赵腮宝[1] 倪开诚[1] 陈文荣[1] 辛德东[1] 郭卫东[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《园艺学报》2012年第7期1253-1262,共10页Acta Horticulturae Sinica

基　　金：浙江省科技重大专项(2007C12021);浙江省科技计划公益技术研究项目(2010C32041);浙江省重点科技创新团队项目(2011R09033-06)

摘　　要：以中国樱桃的优良品种‘短柄樱桃’(Prunus pseudocerasus Lindl.‘Duanbing’)的花芽为材料,采用mRNA 差异显示技术,分析休眠解除的过程中相关差异表达基因。共克隆获得79个差异cDNA片段;通过半定量RT-PCR验证,确定18个阳性差异cDNA片段为休眠解除相关候选基因;休眠解除过程中上调的基因片段11个,下调的7个。测序及同源性比对结果显示:差异表达基因主要参与糖代谢、信号转导、跨膜转运及氧化还原等反应过程。这些基因在‘短柄樱桃’休眠解除阶段可能起到直接或间接的作用。The mRNA differential display technique （DDRT） was used to identify the differentially expressed eDNA fragments during chilling-induced release of dormancy in buds of Prunus pseudocerasus Lindl. ＇Duanbing＇ . Seventy-nine differentially expressed fragments were initially identified by DDRT, and among which, 18 of them were confirmed as true differentially expressed genes using semi- quantitative RT-PCR. Out of these 18 differentially expressed genes, 11 were up-regulated and 7 were down-regulated, respectively, during the release of dormancy. Gene ontology analysis indicated that the differentially expressed genes are mostly involved in the processes of glycometabolism, signal transduction, trans-membrane transport, and oxidation-reduction reactions. The results suggest that these genes may somehow contribute to the release of dormancy, either directly or indirectly, in Prunus pseudocerasus Lindl. ＇Duanbing＇ .

关 键 词：中国樱桃 休眠 基因 MRNA差异显示技术 半定量RT-PCR 

分 类 号：S662.5[农业科学—果树学]